Int Neurourol J.  2015 Sep;19(3):158-163. 10.5213/inj.2015.19.3.158.

Increased Expression of Neuregulin 1 and erbB2 Tyrosine Kinase in the Bladder of Rats With Cyclophosphamide-Induced Interstitial Cystitis

• Song KH
• Youn CS
• Lee CL
• Yang SW
• Chang YS
• Jeong SW
• Sul CK

• KMID: 2380844
• DOI: http://doi.org/10.5213/inj.2015.19.3.158

Abstract

PURPOSE
The aim of this study was to evaluate changes in expressions of neuregulin (NRG)1 and erbB2 tyrosine kinase (ErbB2) in bladders of rats with cyclophosphamide (CYP)-induced interstitial cystitis (IC).
METHODS
Twenty-four Sprague-Dawley rats were divided into the IC group (n=16) and the control group (n=8). After inducing IC with intraperitoneal CYP injection, expressions of NRG1 and ErbB2 were analyzed using western blotting and reverse transcriptase-polymerase chain reaction.
RESULTS
In Western blotting, relative intensities and distributions of both NRG1 and ErbB2 were approximately 1.5- and 3.2-fold higher, respectively, in the IC group than in the control group (mean+/-standard deviation: 1.42+/-0.09 vs. 0.93+/-0.15 and 0.93+/-0.16 vs. 0.29+/-0.08, P<0.05). In the rat bladder samples, mRNA expression levels of NRG1 and ErbB2 were higher in the IC group than in the control group (P<0.05).
CONCLUSIONS
Our study has demonstrated significant changes in mRNA expression and immunoreactivity of NRG1 and ErbB2 receptors in the urinary bladder after CYP-induced IC. These results suggest that the up-regulated NRG1 may play a role in inducing an overactive bladder and promoting regeneration in the inflammatory bladder with CYP-induced IC.

Keyword

Neuregulin-1; Receptor, ErbB-2; Cystitis, Interstitial; Rats

MeSH Terms

Animals
Blotting, Western
Cyclophosphamide
Cystitis, Interstitial*
Neuregulin-1*
Protein-Tyrosine Kinases*
Rats*
Rats, Sprague-Dawley
Receptor, erbB-2
Regeneration
RNA, Messenger
Tyrosine*
Urinary Bladder*
Urinary Bladder, Overactive
Cyclophosphamide
Neuregulin-1
Protein-Tyrosine Kinases
RNA, Messenger
Receptor, erbB-2
Tyrosine