Int Neurourol J.
2012 Dec;16(4):169-174.
Expression of Caveolin-1 in Rat Urinary Bladder with Cyclophosphamide-Induced Cystitis
- Affiliations
-
- 1Department of Urology, Chonnam National University Hospital, Chonnam National University Medical School, Gwangju, Korea. seinsena@hanmail.net
- 2Department of Dermatology, Chonnam National University Hospital, Chonnam National University Medical School, Gwangju, Korea.
- 3Department of Biochemistry, Chonnam National University Hospital, Chonnam National University Medical School, Gwangju, Korea.
Abstract
- PURPOSE
The purposes of this study were to investigate the effect of cyclophosphamide (CYP)-induced inflammatory cystitis on caveolin 1 in rat urinary bladder and to determine the role of these molecules in the bladder dysfunction that occurs in inflammatory change in rat urinary bladder.
METHODS
Female Sprague-Dawley rats were divided into control (n=30) and experimental (n=30) groups. Cystitis in experimental group was induced by intraperitoneal injection of CYP (200 mg/kg). The control group underwent an intraperitoneal saline injection. After 3 days, urodynamic studies were done to measure the contraction interval and contraction pressure. The expression and cellular localization of caveolin 1 were determined by Western blot and immunofluorescent study in rat urinary bladder.
RESULTS
In cystometrograms, the contraction interval (minute) was significantly increased in the CYP-induced cystitis rats (15.8+/-1.5) than in the control group (6.3+/-0.5) (P<0.05). Conversely, the average contraction pressure (mmHg) was significantly higher in the CYP-induced cystitis rats (15.6+/-1.7) than in the control group (11.3+/-0.5) (P<0.05). Caveolin 1 was expressed in the capillaries, arteriols and venules. The protein expression of caveolin 1 was significantly decreased in the CYP-induced cystitis rats (P<0.05).
CONCLUSIONS
Inflammatory change of urinary bladder maybe causes a significant change in the expression of caveolin 1. These findings suggest that caveolin 1 might have a functional role in the bladder dysfunction related with cystitis in rat urinary bladder.
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