Urodynamic Study in Ovalbumin-Induced Cystitis of Rat

Suh, Moo Young; Kim, Young Soo

Abstract

Interstitial cystitis is a chronic inflammatory disease of the bladder characterized symptomatically by pain, urgency and frequent urination due to a small capacity bladder. The etiology of interstitial cystitis is still unknown. Currently, popular theories are that there are a defective bladder epithelium with loss of the blood-urine barrier and autoimmune response to bladder associated antigen. We have used the sensitized rat model to study the effect of the inflammation on bladder function using cystometry and histology Rats were sensitized by intraperitoneal injection of ovalbumin (10mg/ml/kg) given on days 1, 3 and 5. The experiments were performed 4weeks after last injection. Sensitized rats were treated with intravesical ovalbumin instillation for two hours (antigen challenge) and controls were run simultaneously with the sensitized rats by normal saline. In cystometrical investigations, sensitized group showed decreased bladder capacity and low compliance, compared to those of control group. In histologic study, submucosal vessels were more prominent and dilated, and the urothelium was atrophic in sensitized group compared to that in controls group. These results indicate that this experimental cystitis expresses bladder function and histological feature similar to interstitial cystitis of human, which may be resulted from the degeneration of urothelium and detrusor muscle in bladder.