Biomol Ther.  2015 Nov;23(6):539-548. 10.4062/biomolther.2015.043.

EP2 Induces p38 Phosphorylation via the Activation of Src in HEK 293 Cells

• Chun KS
• Shim M

• KMID: 2380305
• DOI: http://doi.org/10.4062/biomolther.2015.043

Abstract

Prostaglandin E2 (PGE2), a major product of cyclooxygenase, binds to four different prostaglandin E2 receptors (EP1, EP2, EP3, and EP4) which are G-protein coupled transmembrane receptors (GPCRs). Although GPCRs including EP receptors have been shown to be associated with their specific G proteins, recent evidences suggest that GPCRs can regulate MAPK signaling via non-G protein coupled pathways including Src. EP2 is differentially expressed in various tissues and the expression of EP2 is induced by extracellular stimuli. We hypothesized that an increased level of EP2 expression may affect MAPK signaling. The overexpression of EP2 in HEK 293 cells resulted in significant increase in intracellular cAMP levels response to treatment with butaprost, a specific EP2 agonist, while overexpression of EP2 alone did not increase intracellular cAMP levels. However, EP2 overexpression in the absence of PGE2 induced an increase in the level of p38 phosphorylation as well as the kinase activity of p38, suggesting that up-regulation of EP2 may promote p38 activation via non-G protein coupled pathway. Inhibition of Src completely blocked EP2-induced p38 phosphorylation and overexpression of Src increased the level of p38 phosphorylation, indicating that Src is upstream kinase for EP2-induced p38 phosphorylation. EP2 overexpression also increased the Src activity and EP2 protein was co-immunoprecipitated with Src. Furthermore, sequential co-immunoprecipitation studies showed that EP2, Src, and beta-arrestin can form a complex. Our study found a novel pathway in which EP2 is associated with Src, regulating p38 pathway.

Keyword

EP2; Prostagladin; p38; beta-arrestin; GPCR

MeSH Terms

Dinoprostone
GTP-Binding Proteins
HEK293 Cells*
Immunoprecipitation
Phosphorylation*
Phosphotransferases
Prostaglandin-Endoperoxide Synthases
Up-Regulation
Dinoprostone
GTP-Binding Proteins
Phosphotransferases
Prostaglandin-Endoperoxide Synthases