Exp Mol Med.  2017 Mar;49(3):e308. 10.1038/emm.2017.22.

Next-generation sequencing enables the discovery of more diverse positive clones from a phage-displayed antibody library

Affiliations
  • 1Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, Korea. jjhchung@snu.ac.kr
  • 2Department of Cancer Biology, Seoul National University College of Medicine, Seoul, Korea.
  • 3Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea.
  • 4Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT, USA.

Abstract

Phage display technology provides a powerful tool to screen a library for a binding molecule via an enrichment process. It has been adopted as a critical technology in the development of therapeutic antibodies. However, a major drawback of phage display technology is that because the degree of the enrichment cannot be controlled during the bio-panning process, it frequently results in a limited number of clones. In this study, we applied next-generation sequencing (NGS) to screen clones from a library and determine whether a greater number of clones can be identified using NGS than using conventional methods. Three chicken immune single-chain variable fragment (scFv) libraries were subjected to bio-panning on prostate-specific antigen (PSA). Phagemid DNA prepared from the original libraries as well as from the Escherichia coli pool after each round of bio-panning was analyzed using NGS, and the heavy chain complementarity-determining region 3 (HCDR3) sequences of the scFv clones were determined. Subsequently, through two-step linker PCR and cloning, the entire scFv gene was retrieved and analyzed for its reactivity to PSA in a phage enzyme immunoassay. After four rounds of bio-panning, the conventional colony screening method was performed for comparison. The scFv clones retrieved from NGS analysis included all clones identified by the conventional colony screening method as well as many additional clones. The enrichment of the HCDR3 sequence throughout the bio-panning process was a positive predictive factor for the selection of PSA-reactive scFv clones.


MeSH Terms

Antibodies
Bacteriophages
Chickens
Clone Cells*
Cloning, Organism
Complementarity Determining Regions
DNA
Escherichia coli
Immunoenzyme Techniques
Mass Screening
Methods
Polymerase Chain Reaction
Prostate-Specific Antigen
Single-Chain Antibodies
Antibodies
Complementarity Determining Regions
DNA
Prostate-Specific Antigen
Single-Chain Antibodies
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