J Gastric Cancer.  2017 Mar;17(1):43-51. 10.5230/jgc.2017.17.e5.

Analysis of G3BP1 and VEZT Expression in Gastric Cancer and Their Possible Correlation with Tumor Clinicopathological Factors

• Beheshtizadeh M
• Moslemi E

• KMID: 2372581
• DOI: http://doi.org/10.5230/jgc.2017.17.e5

Abstract

PURPOSE
This study aimed to analyze G3BP1 and VEZT expression profiles in patients with gastric cancer, and examine the possible relationship between the expressions of each gene and clinicopathological factors.
MATERIALS AND METHODS
Expression of these genes in formalin-fixed paraffin embedded (FFPE) tissues, collected from 40 patients with gastric cancer and 40 healthy controls, was analyzed. Differences in gene expression among patient and normal samples were identified using the GraphPad Prism 5 software. For the analysis of real-time polymerase chain reaction products, GelQuantNET software was used.
RESULTS
Our findings demonstrated that both VEZT and G3BP1 mRNA expression levels were downregulated in gastric cancer samples compared with those in the normal controls. No significant relationship was found between the expression of these genes and gender (P-value, 0.4835 vs. 0.6350), but there were significant changes associated with age (P-value, 0.0004 vs. 0.0001) and stage of disease (P-value, 0.0019 vs. 0.0001). In addition, there was a direct relationship between VEZT gene expression and metastasis (P-value, 0.0462), in contrast to G3BP1 that did not demonstrate any significant correlation (P-value, 0.1833).
CONCLUSIONS
The results suggest that expression profiling of VEZT and G3BP1 can be used for diagnosis of gastric cancer, and specifically, VEZT gene could be considered as a biomarker for the detection of gastric cancer progression.

Keyword

Stomach neoplasms; G3BP1; VEZT; Gene expression

MeSH Terms

Diagnosis
Gene Expression
Humans
Neoplasm Metastasis
Paraffin
Real-Time Polymerase Chain Reaction
RNA, Messenger
Stomach Neoplasms*
Paraffin
RNA, Messenger

Figure

• 

  Fig. 1 Melting curves of (A) GAPDH, (B) VEZT, and (C) G3BP1 genes in normal and cancerous samples show specific qPCR products for each gene. qPCR = quantitative polymerase chain reaction.

• 

  Fig. 2 VEZT and G3BP1 gene expression mRNA level in TNM calssification. The achieved results were analyzed with GraphPad Prism 5 (GraphPad Software, Inc., La Jolla, CA, USA). Each experiment was repeated 3 times. The P-value for VEZT and G3BPI are 0.0019 and 0.0001, respectively. TNM = tumor, node, metastasis; RQ = relative quantitation.

• 

  Fig. 3 G3BP1 and GAPDH real-time PCR products were loaded in upper and lower lane, respectively. The results obtained after GelQuantNET software (BiochemLabSolutions, San Francisco, CA, USA) and GraphPad Prism 5 analysis (GraphPad Software, Inc., La Jolla, CA, USA), suggest no significant correlation between the G3BP1 expression and disease progression (P-value, 0.0886), while VEZT expression was found to be significantly correlated (P-value, 0.0175). PCR = polymerase chain reaction.

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