Korean J Vet Res.  2016 Mar;56(1):29-35. 10.14405/kjvr.2016.56.1.29.

Protective effect of phloroglucinol against gamma radiation-induced oxidative stress in hair follicles

• Kim A
• Bing SJ
• Cho J
• Herath K
• Jeon YJ
• Lee BG
• Park JW
• Jee Y

• KMID: 2368738
• DOI: http://doi.org/10.14405/kjvr.2016.56.1.29

Abstract

When exposed to gamma-rays, hair follicular cells immediately go through apoptosis, which hampers their rapid differentiation essential for the regeneration of hair. Phloroglucinol (PG) is a phenolic compound of Ecklonia cava, brown algae abundant in Jeju island, Korea. Containing plentiful polyphenols, PG is known for its instructive effects by inhibiting apoptosis, scavenging oxygen radicals, and protecting cells against oxidative stress. In this study, we demonstrate that PG rescues radiosensitive hair follicular cells from gamma radiation-induced apoptosis and DNA damage. To identify protective capacity of PG on hair follicles, we irradiated with 8.5 Gy (1.5 Gy/min) of gamma-rays to the whole body of C57BL/6 mice at day 6 after depilation with or without PG. In mice exposed to radiation, the expression of proapoptotic molecule p53 was downregulated in the skin of PG treated group. On immunohistochemical observation of the skin, PG inhibited the immunoreactivity of p53 and cleaved caspase-3. PG treatment protected hair follicular cells from cell death due to gamma-radiation. Our results suggest that PG presents radioprotective effects by inhibiting apoptosis of radiosensitive hair follicular cells and can protect hair follicular cells from gamma-ray induced damage.

Keyword

gamma-ray; hair follicular cells; oxidative stress; phloroglucinol; radioprotection

MeSH Terms

Animals
Apoptosis
Caspase 3
Cell Death
DNA Damage
Hair Follicle*
Hair Removal
Hair*
Korea
Mice
Oxidative Stress*
Phaeophyta
Phenol
Phloroglucinol*
Polyphenols
Reactive Oxygen Species
Regeneration
Skin
Caspase 3
Phenol
Phloroglucinol
Polyphenols
Reactive Oxygen Species