Optimization of the mixed ratio of organic tangerine peel and guarana extracts to suppress fat accumulation

Lee, Hyun A; Hong, Sunhwa; Chung, Yungho; Kim, Okjin

Abstract

Hyperlipidemia has been ranked as one of the greatest risk factors contributing to the prevalence and severity of coronary heart diseases. The pharmacological actions of tangerine (Citrus unshiu) peel include the facilitation of fat digestive enzymes. Also, guarana (Paullinia cupana) has been used for stimulants and tonics over a long period. In this study, we aimed to optimize the mixed ratio of organic tangerine peel and guarana extracts to suppress fat accumulation. To determine the optimized the mixed ratio of tangerine (Citrus unshiu) peel extract (C) and guarana (Paullinia cupana) extract (P) on adipogenesis, maturing preadipocytes were incubated during the 8-day induction period with various ratio of the mixed extracts groups like as Vehicle (DMEM 200 µl/ml), Con (MDI DMEM 200 µl/ml), C10 (MDI DMEM 180µl/ml+C 20 µl/ml), C9:P1 (MDI DMEM 180 µl/ml+C 18 µl/ml+P 2 µl/ml), C5:P5 (MDI DMEM 180 µl/ml+C 10 µl/ml+P 10 µl/ ml), P10 (MDI DMEM 180 µl/ml+P 20 µl/ml). Thereafter, the adipocytes were stained with Oil-Red-O and analyzed for lipid contents. As the results, organic tangerine peel and guarana extracts were revealed to reduce fat accumulation in 3T3-L1 cells. The fat accumulation significantly decreased in C5:P5 group, which is equally mixed with organic tangerine peel and guarana extracts, as compared to other groups. Based on these results, we found the optimized ratio with organic tangerine peel and guarana extracts to suppress fat accumulation. We suggest that this optimized organic tangerine peel and guarana complex might reduce effectively the serum lipid components and improve the lipid metabolism in hyperlipidemic patient.