J Korean Acad Oral Health.  2019 Sep;43(3):131-135. 10.11149/jkaoh.2019.43.3.131.

Effects of Diospyros kaki peel, Momordica charantia, and Canavalia gladiata extracts on the cariogenic traits of Streptococcus mutans

• Kim DW
• Lee DI
• Im SU
• Choi YH
• Song KB

• KMID: 2459410
• DOI: http://doi.org/10.11149/jkaoh.2019.43.3.131

Abstract

OBJECTIVES
The purpose of this study is to determine methods of dental caries prevention by investigating the use of compounds of Diospyros kaki (D. kaki) peel, Momordica charantia (M. charantia), and Canavalia gladiata (C. gladiata) extracts to limit the cariogenic traits of Streptococcus mutans (S. mutans), such as their ability to proliferate and adhere to the tooth surface.
METHODS
Broth microdilution and the agar spreading assay were used to determine the antimicrobial effect and minimum inhibitory concentration (MIC) of S. mutans extracts. In order to identify the adhesive ability of S. mutans at varying concentrations, culture plates were first stained with 1 ml of 0.01% crystal violet for 15 minutes at room temperature, and then eluted with 1 ml of EtOH:Acetone (8:2) solution for 15 minutes in a 37℃ incubator. Eluted solutions were then evaluated by use of a spectrophotometer at 575 nm.
RESULTS
Experiments were conducted in order to investigate the effectiveness of D. kaki peel, M. charantia, and C. gladiata extracts on limiting the proliferation of S. mutans. The MIC was measured as an indication of whether the antibacterial activity of D. kaki peel, M. charantia, and C. gladiata extracts had a significant bacteriostatic effect on S. mutans. M. charantia extract was effective for growth inhibition on S. mutans at a minimum concentration of 0.25%. From the adhesion ability assay, M. charantia extract had an anti-adhesive effect.
CONCLUSIONS
These results indicate that M. charantia extract demonstrates antibacterial activity and has an anti-adhesive effect on S. mutans. Due to these properties, M. charantia extract may be used to prevent dental caries.

Keyword

Antibacterial activity; Anti-adhesive effect; Canavalia gladiata; Diospyros kaki peel; Momordica charantia; Streptococcus mutans

MeSH Terms

Adhesives
Agar
Canavalia*
Dental Caries
Diospyros*
Gentian Violet
Incubators
Microbial Sensitivity Tests
Momordica charantia*
Momordica*
Streptococcus mutans*
Streptococcus*
Thiram
Tooth
Adhesives
Agar
Gentian Violet
Thiram

Figure

• 

  Fig. 1. Adhesive ability of S. mutans with different concentrations of several extracts.

Reference

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