Clin Exp Otorhinolaryngol.  2016 Dec;9(4):352-357. 10.21053/ceo.2015.01648.

Age-Related Changes in Antioxidative Enzyme Capacity in Tongue of Fischer 344 Rats

• Baek MK
• Kim KO
• Kwon HJ
• Kim YW
• Woo JH
• Kim DY

• KMID: 2360767
• DOI: http://doi.org/10.21053/ceo.2015.01648

Abstract

OBJECTIVES
Antioxidative enzyme efficiency changes in some organs with age. However, no study has been conducted on age-related antioxidant enzyme changes in tongue. In the present study, the authors investigated the activities of four antioxidative enzymes and their protein expressions in the tongues of young and old Fischer 344 rats.
METHODS
Age-dependent changes in the enzyme activities of total superoxide dismutase (SOD), Mn-SOD, Cu/Zn-SOD, catalase (CAT), and glutathione peroxidase (GPx) were determined using chemical kits, and the protein expressions levels of these enzymes by Western blotting. The study was conducted using rats aged 7 months (the young group, n=8) and 22 months (the old group, n=8).
RESULTS
Total SOD, Cu/Zn-SOD, and GPx activities in the tongues of old rats were lower than in young rats, and similarly, corresponding protein expressions were downregulated in old rats. On the other hand, although the protein expressions of Mn-SOD and CAT were lower in old rats, their enzyme activities were not.
CONCLUSION
The results of this study provide a possible mechanism for the tongue aging process, as in old Fischer 344 rats the antioxidant defense system was diminished with respect to enzyme activity levels and protein abundances.

Keyword

Antioxidants; Tongue; Aging; Oxidative Stress

MeSH Terms

Aging
Animals
Antioxidants
Blotting, Western
Catalase
Cats
Glutathione Peroxidase
Hand
Oxidative Stress
Rats*
Superoxide Dismutase
Tongue*
Antioxidants
Catalase
Glutathione Peroxidase
Superoxide Dismutase

Figure

• 

  Fig. 1. (A) Total SOD, (B) Mn-SOD, (C) Cu/Zn-SOD, (D) GPx, and (E) CAT activities in the rats’ tongues of two age groups, namely 7 months (young group, n=8) and 22 months (old group, n=8). One unit of SOD is defined as the amount of enzyme needed to catalyze the dismutation of the superoxide (O2−) radical by 50% at 25°C. One unit of GPx was defined as the amount of enzyme required to cause the oxidation of 1.0 nmol of NADPH to NADP+ per minute at 25°C. One unit of CAT was defined as the amount of enzyme required to produce 1.0 nmol of formaldehyde per minute at 25°C. Results are expressed as median values. SOD, superoxide dismutase; GPx, glutathione peroxidase; CAT, catalase; NADP, nicotinamide adenine dinucleotide phosphate. a)P<0.05. b)P<0.01 vs. the young group by the Mann-Whitney test.

• 

  Fig. 2. Western blot analysis of antioxidant enzyme protein expressions in the rats’ tongues of two age groups, namely 7 months (young group, n=8) and 22 months (old group, n=8). Protein (20 ug) lysates from tongue homogenates of young and old rats were resolved by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel) and probed with Mn-SOD, Cu/Zn-SOD, CAT, and GPx antibodies as described in Materials and Methods. GAPDH was used as a loading control. Blots of (A) Mn-SOD, (B) Cu/Zn-SOD, (C) GPx, and (D) CAT were shown with quantification of the protein expression by normalizing each band signal to their corresponding GAPDH band. Results are expressed as median values. SOD, superoxide dismutase; GPx, glutathione peroxidase; CAT, catalase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. a)P<0.05. b)P<0.01 vs. the young group by the Mann-Whitney test.

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