Ann Dermatol.  1997 Oct;9(4):246-252. 10.5021/ad.1997.9.4.246.

Early Phase of UVB-induced GM-CSF Upregulation in Epithelial Cell Line is not Totally Dependent on IL-1α

• Park KC
• Whang JH
• Youn SW
• Ahn JS
• Kim YG
• Koh WS
• Kim KH
• Chung JH
• Youn JI
• Jung HC

• KMID: 2360347
• DOI: http://doi.org/10.5021/ad.1997.9.4.246

Abstract

BACKGROUND
It was demonstrated that ultraviolet(UV) B light induces the release of IL-1α in cultured human epithelial cell line and augmentation of GM-CSF production by UVB is reported to be mediated by IL-1α in the murine keratinocyte cell line Pam 212.
OBJECTIVE
The purpose of this study was to evaluate the effects of UVB on kinetic profile of IL-1 and GM-CSF mRNA expression and to see whether synthesis of GM-CSF by UVB can be completely inhibited by blocking IL-1α mediated pathway.
METHODS
We used a competitive RT-PCR for measuring cytokine gene expression in epithelial cell line after UV radiation.
RESULTS
The IL-1α mRNA increased as early as 1h after UV irradiation, and then decreased at 3h after the irradiation. Thereafter, the response of IL-1α mRNA was upregulated with a second peak at 6h after the UV irradiation. However, mRNA for GM-CSF increased at 1h after UV light exposure and anti-IL-1α antibodies could only partially inhibit UV-augmented GM- CSF production.
CONCLUSION
UVB induced GM-CSF production seemed to be mainly mediated by UVB induced IL-1α but these results suggest that UVB may also induce GM-CSF production through an IL-1α independent pathway.

Keyword

Ultraviolet B; IL-1α; GM-CSF; RT-PCR

MeSH Terms

Antibodies
Cell Line
Epithelial Cells*
Gene Expression
Granulocyte-Macrophage Colony-Stimulating Factor*
Humans
Interleukin-1
Keratinocytes
RNA, Messenger
Ultraviolet Rays
Up-Regulation*
Antibodies
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-1
RNA, Messenger