J Korean Acad Periodontol.  1997 Dec;27(4):805-818. 10.5051/jkape.1997.27.4.805.

Effects of Substance P on the Cell Proliferation and IL-2 Production of T Lymphocyte

• Moon JK
• Choi BS
• Lee SC
• Kim HS

• KMID: 2057009
• DOI: http://doi.org/10.5051/jkape.1997.27.4.805

Abstract

Immune responses of periodontal tissue may be regulated by products of sensory afferent nerve endings such as neuropeptides. Substance P(SP), a tachykinin neuropeptide, has been previously reported to stimulate the activities of T lymphocyte. Therefore, I examined the role of SP in IL-2 production and cell proliferation by using a homogeneous line of T lymphocytes(Jurkat and HuT78). Cell proliferation rate was determined by [3H]-thymidine incorporation test, and IL-2 was quantitated by the growth rate of CD4+ IL-2-dependent T lymphocyte line CTLL-2. SP stimulated cell proliferation of T lymphocytes at the concentration of 10(-12) and 10(-8)M in a biphasic bell-shape dose-dependent manner. However, SP alone did not induce IL-2 release at the concentration range of 10(-6) to 10(-14)M. The upregulation of IL-2 release was observed when 10(-12)M SP was applied together with mitogens such as Con A or PHA+PMA on T cell lines, especially on Jurkat. Con A or PHA+PMA demonstrated to increase the rate of cell proliferation of Jurkat, which had shown to produce much amount of IL-2 indicating that mitogen-induced cell proliferation might be partially influenced by released IL-2. It was concluded that regulatory effects of SP on the immune/inflammatory response could be mediated through the costimulatory upregulation of IL-2 production and increase of cell proliferation of T lymphocyte.

MeSH Terms

Cell Line
Cell Proliferation*
Interleukin-2*
Lymphocytes*
Mitogens
Nerve Endings
Neuropeptides
Substance P*
T-Lymphocytes
Tachykinins
Up-Regulation
Interleukin-2
Mitogens
Neuropeptides
Substance P
Tachykinins