J Korean Dent Soc Anesthesiol.
2014 Jun;14(2):107-114. 10.17245/jkdsa.2014.14.2.107.
Effect of Propofol Preconditioning on Hypoxic-Cultured Human Osteoblast
- Affiliations
-
- 1Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University, Gyeongnam, Korea. shinsw@pusan.ac.kr
- 2Department of Oral Anatomy, School of Dentistry, Pusan National University, Gyeongnam, Korea.
- 3Department of Dental Anesthesia and Pain Medicine, Pusan National University Dental Hospital, Gyeongnam, Korea.
- 4Department of Dental Anesthesia and Pain Medicine, School of Dentistry, Pusan National University, Gyeongnam, Korea.
- KMID: 2344996
- DOI: http://doi.org/10.17245/jkdsa.2014.14.2.107
Abstract
- BACKGROUND
Angiogenesis has been recognized an essential precondition for osteogenesis. Because reduction and disruption of the blood supply to tissue cause tissue hypoxia, pathological bone loss affected by hypoxia often can occur in various clinical conditions. The effects of propofol on the process of osteogenesis have received little direct attention. Therefore, we investigated the effect of propofol on the growth and function of osteoblasts under hypoxic condition.
METHODS
After propofol (3, 30, 300 µM) preconditioning for 2 hours, hFOB 1.19 human osteoblast cells were cultured under 1 % oxygen tension for 48 hours. Using real time PCR and western blot analysis, we analyzed the expression of, BMP-2, TGF-β1, type I collagen, osteocalcin, HIF-1s and Akt. Cell viability was also determined by MTT assay.
RESULTS
Propofol preconditioning on hypoxic-cultured osteoblast promoted the expressions of BMP-2, TGF-β1, type I collagen and osteocalcin and induced hypoxia-mediated HIF-1 activation and the expression of Akt protein. Propofol with 300 µM significant decreased cell viability compared to control.
CONCLUSIONS
Clinically relevant concentrations of propofol are not cytotoxic to hypoxic osteoblasts in vitro. Propofol preconditioning on hypoxic-cultured osteoblast stimulates proliferation and differentiation of osteoblast through induced expression of BMP-2, TGF-β1, type I collagen and osteocalcin. Propofol might promote angiogenesis and bone regeneration under hypoxic condition.
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