J Korean Neuropsychiatr Assoc.  2000 Sep;39(5):928-935.

A New Gene Regulated by Electroconvulsive Shock in Rat Brains

• Chung SJ
• Park JE
• Kang UG
• Koo YJ
• Park JB
• Kim YS

Abstract

OBJECTIVES
This study was performed to identify genes regulated by electroconvulsive shock (ECS) and to observe the pattern of expression of genes according to different developmental stages and brain regions.
METHODS
ECS(130V, 0.5 sec) was given to male Sprague-Dawley rats with age of postnatal day 7 and 21(P7, P21 respectively). After screening genes regulated by ECS with mRNA differential display-PCR(DD-PCR), we selected one clone among them and observed the induction of this gene after ECS by time-dependent Northern blot analysis of rat brain of P7, P21 and adult rat cortex and hippocampus.
RESULTS
By DD-PCR method, we have identified four clones whose expression was regulated by ECS. Among them, one(CP 10-2) was proved to be a new gene by sequencing and BLAST search. Its expression was increased after ECS in P7, P21, and adult rat brain. The expression of CP 10-2 reached peak level at 180 minutes after ECS in P7 rat brain, but was further increased until 360 minutes after ECS in P21 and adult rat brain.
CONCLUSION
In this study, a new gene was identified in rat brain which showed up-regulated expression in response to ECS. Cloning and characterization of this new gene would be helpful to elucidate the effect of ECS in rat brain.

Keyword

Electroconvulsive shock; Differential display-PCR; Gene; Rat brain

MeSH Terms

Adult
Animals
Blotting, Northern
Brain*
Clone Cells
Cloning, Organism
Electroshock*
Hippocampus
Humans
Male
Mass Screening
Rats*
Rats, Sprague-Dawley
RNA, Messenger
RNA, Messenger