J Korean Soc Coloproctol.  2000 Oct;16(5):285-292.

Expression of MMP-2 (Matrix Metalloproteinase Type 2) and TIMP-2 (Tissue Inhibitor of Metalloproteinase Type 2) in Color

• Baek MJ
• Chu CW
• Chae MK
• Kim SY
• Lee MS
• Kim CH
• Kim DJ
• Hwang KY
• Song OP

Abstract

PURPOSE: The matrix metalloproteinases (MMPs) have been implicated in proteolysis of basement membrane for initiation of metastatic cascade. Tissue inhibitors of metalloproteinases (TIMPs) are specific inhibitors of MMPs. The purpose of this study was to evaluate the expression of MMP-2 and TIMP-2 in human colorectal carcinomas.
METHODS
The paraffin blocks of 140 colorectal carcinomas were recalled and immunostained with monoclonal antibodies specific for MMP-2 and TIMP-2. These antibodies were effective on formalin fixed, paraffin embedded sections. The rate of stain was estimated, and the relationships between the expression and the stage, the differentiation, lymph node metastasis, distant metastasis and the survival rate were assessed.
RESULTS
MMP-2 was present in 31.4% of colorectal cancers. TIMP-2 was identified in 63.6% of tumors. The expression of MMP-2 was significantly associated with the presence of lymph-node metastasis, the stage, and the presence of distant metastasis. However the expression of TIMP-2 was not correlated with any risk factors.
CONCLUSIONS
These results suggest that MMP-2 could predict the ability of cancer invasion and be used as a prognostic factor for the colorectal adenocarcinoma.

Keyword

Colorectal cancer; MMP-2; TIMP-2

MeSH Terms

Adenocarcinoma
Antibodies
Antibodies, Monoclonal
Basement Membrane
Colorectal Neoplasms
Formaldehyde
Humans
Lymph Nodes
Matrix Metalloproteinases
Metalloproteases
Neoplasm Metastasis
Paraffin
Proteolysis
Risk Factors
Survival Rate
Tissue Inhibitor of Metalloproteinase-2*
Antibodies
Antibodies, Monoclonal
Formaldehyde
Matrix Metalloproteinases
Metalloproteases
Paraffin
Tissue Inhibitor of Metalloproteinase-2