J Korean Med Sci.  2000 Jun;15(3):309-314. 10.3346/jkms.2000.15.3.309.

Matrix metalloproteinases in human gliomas: activation of matrix metalloproteinase-2 (MMP-2) may be correlated with membrane-type-1 matrix metalloproteinase (MT1-MMP) expression

• Hur JH
• Park MJ
• Park IC
• Yi DH
• Rhee CH
• Hong SI
• Lee SH

• KMID: 1128630
• DOI: http://doi.org/10.3346/jkms.2000.15.3.309

Abstract

To evaluate possible roles of matrix metalloproteinase (MMP)-1, -2, tissue inhibitor of metalloproteinase (TIMP)-1, -2 and membrane-type-1 matrix metalloproteinase (MT1-MMP) in invasion of human gliomas, expressions of these proteins were investigated in ten cases of human glioma and two meningioma tissues and eight human glioma cell lines. In gelatin zymography, MMP-2 activities of glioblastomas were higher than astrocytomas. The activated form of MMP-2 was seen in five of six cases of glioblastomas, but not in astrocytomas. MMP-9 activity was detected in all cases of malignant astrocytomas but the reactivity of MMP-9 was weaker than that of MMP-2. MT1-MMP mRNA expression in glioblastomas was higher than that in astrocytomas. Five cases of glioblastomas with activated form of MMP-2 had MT1-MMP expressions. In vitro, human glioma cell lines with high expression of MT1-MMP also showed high MMP-2 activity. TIMP-1 transcripts were constitutively present in almost all glioma tissues and cell lines, whereas TIMP-2 mRNA were weak especially in malignant gliomas. Imbalance of TIMP-2/MMP-2 was observed using immunoprecipitation analysis in a glioma cell line. High expression of MMP-2 and MT1-MMP is possibly involved in invasiveness of malignant glioma.

Keyword

Glioma; Matrix Metalloproteinase (MMPs); Tissue Inhibitor of Metalloproteinase (TIMPs); Membrane-Type-1 Metalloproteinase (MT1-MMP); Neoplasms Invasiveness

MeSH Terms

Animal
Blotting, Northern/methods
Brain/pathology
Brain Neoplasms/pathology
Brain Neoplasms/enzymology*
Enzyme Activation
Gelatinase A/metabolism
Gelatinase A/genetics*
Gelatinase B/metabolism
Gene Expression Regulation, Enzymologic*
Glioma/pathology
Glioma/enzymology*
Human
Metalloendopeptidases/genetics*
Papio
Tissue Inhibitor-of Metalloproteinase-2/genetics
Tissue-Inhibitor of Metalloproteinase-1/genetics
Tumor Cells, Cultured