J Korean Continence Soc.  2009 Dec;13(2):134-141.

Proteomic Changes in the Female Rat Bladder Tissue following Bilateral Oophorectomy

• Lee HC
• Kim HJ

Abstract

PURPOSE
To explore the effect of bilateral oophorectomy on the several proteins of female rat bladder via a proteomic approach.
MATERIALS AND METHODS
A total of 20 female Sprague-Dawley rats were obtained at 8 weeks of age and were divided randomly into 3 groups: a control groups underwent sham operation and bladders were excised 4 weeks and 8 weeks after operation each. Other two groups underwent bilateral oophorectomy and bladders were excised 4 weeks (group 1) and 8 weeks (group 2) after operation each, too. Each group has 5 rats. Conventional proteomics was conducted via high resolution 2-D gel electrophoresis, followed by image analysis and protein identification through mass spectrometry.
RESULTS
Bladder weights were reduced significantly in group 2 as compared with the 8-week control group. A comparison of the bladders of the oophorectomy group subjects with those of the control group subjects demonstrated that the expressions of 11 proteins were altered Eukaryotic translation initiation factor 5A (elF-5A), chaperone grp 75 precursor, actin-depolymerizing factor, contrapsin-like inhibitor 1 precursor, guanine deaminase, actin, peroxiredoxin 2, phosphatidylethanolamine, putative protein kinase, Keratin complex 2 and Enol protein.
CONCLUSION
The roles of 11 proteins are variable. A part of these proteins have a role of cellular apoptosis, acute inflammation, and muscle contraction. It seems that these changes of proteins have an influence on bladder functions and LUTS.

Keyword

Oophorectomy; Bladder; Proteomics; Protein

MeSH Terms

Actins
Animals
Apoptosis
Electrophoresis, Gel, Two-Dimensional
Female
Guanine Deaminase
Humans
Inflammation
Mass Spectrometry
Muscle Contraction
Ovariectomy*
Peptide Initiation Factors
Peroxiredoxins
Protein Kinases
Proteomics
Rats*
Rats, Sprague-Dawley
Urinary Bladder*
Weights and Measures
Actins
Guanine Deaminase
Peptide Initiation Factors
Peroxiredoxins
Protein Kinases