J Korean Soc Endocrinol.  1997 Dec;12(4):557-564.

Effects of Thyroid Hormone on Preduction of Interleukin-6 and Interleukin-11 in Human Bone Marrow Stromal Cells

• Kim CH
• Kim DK
• Kim HK
• Song YK
• Kim KS

Abstract

BACKGROUND
It is well known that excessive thyroid hormone in the body is associated with bone loss. However, the mechanism by which thyroid hormone affects bone cell metabolism remains unclear. It has been shown that thyroid hormones stimulate osteoclastic bone resorption indirectly via some unknown mediators secreted by osteoblasts, This study was undertaken to determine if interleukin-6 (IL-6) or interleukin-11 (IL-l1) could be the mediator (s) of thyroid hormone-induced bone loss.
METHODS
We treated primary cultured human bone rnarrow stromal cells with 3,5,3-triiodo-thyronine (T) and measured basal and interleukin-l (IL-1)-stimulated IL-6/IL-ll production. We also investigated the possible modulating effect of 17B-estradiol (17B-E2.) on thyroid hormone action.
RESULTS
T3 at 10 (-12) ~ 10 (-8) M concentration, significantly increased the basal IL-6 production in a dose-dependent manner, and also potentiated the stimulatory effect of IL-1 on IL-6 production. However, T failed to elicit a detectable effect on basal or IL-1-stimulated IL-11 production. Treat#ment with l7B-E2. inhibited IL-1-stimulated IL-6 production, but the effects of T3 on IL-6 production were not affected by 17/B-E.
CONCLUSION
These results suggest that thyroid hormone may increase bone resorption by increasing basal IL-6 production and potentiating IL-1-induced IL-6 production from osteoblast-lineage cells, and these effects were independent of estrogen status.

MeSH Terms

Bone Marrow*
Bone Resorption
Estradiol
Estrogens
Humans*
Interleukin-1
Interleukin-11*
Interleukin-6*
Mesenchymal Stromal Cells*
Metabolism
Osteoblasts
Osteoclasts
Osteoporosis
Stromal Cells
Thyroid Gland*
Thyroid Hormones
Estradiol
Estrogens
Interleukin-1
Interleukin-11
Interleukin-6
Thyroid Hormones