J Nutr Health.  2015 Apr;48(2):157-166. 10.4163/jnh.2015.48.2.157.

Effect of water extract and distillate from the mixture of black goat meat and medicinal herb on osteoblast proliferation and osteoclast formation

• Song HN
• Leem KH
• Kwun IS

• KMID: 2327186
• DOI: http://doi.org/10.4163/jnh.2015.48.2.157

Abstract

PURPOSE
The effects of water extract and distillate from the mixture of black goat meat and medicinal herb on MG-63 osteoblast proliferation and mouse bone marrow derived osteoclast formation were investigated.
METHODS
Proximate composition, volatile basic nitrogen (VBN), mineral content, free amino acid composition and free fatty acid composition in black goat meat were determined. Water extract and distillate were prepared with three groups; goat meat only (BG-E, BG-D), six herbs added group (BG-E6, BG-D6), and eight herbs added group (BG-E8, BG-D8). Osteoblast proliferation, mineralization and calcium uptake activity of MG-63 cells were measured and tartrate resistant acid phosphatase activity of osteoclasts was analyzed.
RESULTS
Black goat meat had remarkably low fat and high level of calcium. Glutamic acid was the most abundant amino acid. Herbs added extract groups (BG-E6 and BG-E8) showed increased MG-63 cell proliferation in a concentration dependent manner, while all the distillates did not show the effect. All extracts and distillates showed significantly increased osteoblast mineralization depending on the concentration. In particular, herb added extract, BG-E6, increased 170.3% of control and the distillate of BG-D and BG-D6 increased up to 168.5% and 159.8%, respectively. Calcium uptake activities of all water extracts showed remarkable increase of BG-E6 and BG-E8 up to 615.5% and 628.1% of control, respectively. Ditillates had no effect except BG-D6. All water extracts significantly reduced the activity of tartrate-resistant acid phosphatase (TRAP) in osteoclasts derived from mouse bone marrow.
CONCLUSION
Combination of black goat meat and medicinal herb increased the MG-63 cell proliferation and effectively inhibited osteoclast differentiation in both water extracts and distillate of them, which implies that they could be used as potent functional food materials for bone health.

Keyword

black goat meat; medicinal herb; MG-63 cell; osteoblast; osteoclast

MeSH Terms

Acid Phosphatase
Animals
Bone Marrow
Calcium
Cell Proliferation
Functional Food
Glutamic Acid
Goats*
Meat*
Mice
Nitrogen
Osteoblasts*
Osteoclasts*
Plants, Medicinal*
Water*
Acid Phosphatase
Calcium
Glutamic Acid
Nitrogen
Water

Figure

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  Fig. 1. Scheme of manufacturing black goat extract and its distilled extract

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  Fig. 2. Effect of black goat meat and medicinal herb mixture on proliferation of human osteoblast MG-63 cells. D10x, 1/10 dilution; D80x, 1/80 dilution; D640x, 1/640 dilution. Data shown are the mean and SD values of three independent samples. The treatments sharing a common alphabet are not statistically significant at 5% level.

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  Fig. 3. Effect of black goat meat and medicinal herb mixture on mineralization of human osteoblast MG-63 cells. D10x, 1/10 dilution; D80x, 1/80 dilution. Data shown are the mean and SD values of three independent samples. The treatments sharing a common alphabet are not statistically significant at 5% level.

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  Fig. 4. Effect of black goat meat and medicinal herb mixture on calcium uptake of human osteoblast MG-63 cells. D10x, 1/10 dilution; D80x, 1/80 dilution; D640x, 1/640 dilution. Data shown are the mean and SD values of three independent samples. The treatments sharing a common alphabet are not statistically significant at 5% level.

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  Fig. 5. Effect of black goat meat and medicinal herb mixture on TRAP activity. Data shown are the mean and SD values of three independent samples. The treatments sharing a common alphabet are not statistically significant at 5% level.

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