J Korean Acad Periodontol.  2009 Aug;39(Suppl):269-278.

MMP-1 promoter polymorphism in Korean with generalized aggressive periodontitis

• Oh HS
• Kim OS
• Kim YJ
• Chung HJ

Abstract

PURPOSE: The aim of this study was to investigate matrix metalloproteinase 1 (MMP-1) gene polymorphism (1G/2G at -1607 and A/G at -519) in Korean subject and to assess the association between polymorphism and periodontal status.
METHODS
Forty nine generalized aggressive periodontitis (GAP) patients and 57 periodontally healthy children were recruited and genomic DNA was extracted from buccal swab. The polymorphisms of MMP-1 promoter genes were determined by polymerase chain reaction and restriction fragment length product (PCR-RFLP) method. The distribution of genotype and allele frequency was compared between 2 groups by chi-square test.
RESULTS
There was a significant difference in the distribution of genotypes and frequency of alleles between the GAP and reference groups at the position - 519 of MMP-1 gene promoter (P< 0.05). Allele G carrier rate was significantly lower in GAP group than that of the reference group (P< 0.001). At the position -1607 of MMP-1 gene promoter, genotype distribution and allele frequency showed no statistically significant difference between the groups. However, in the female group, a significant difference was observed between the groups for the genotype distribution, allele frequency and allele 1G carrier rate (P< 0.05).
CONCLUSIONS
The DNA polymorphism at the MMP-1 gene promoter might be associated with GAP in Korean.

Keyword

aggressive periodontitis; genetic polymorphism; matrix metalloproteinase 1

MeSH Terms

Aggressive Periodontitis
Alleles
Child
DNA
Female
Gene Frequency
Genotype
Humans
Matrix Metalloproteinase 1
Polymerase Chain Reaction
Polymorphism, Genetic
DNA
Matrix Metalloproteinase 1

Figure

• 

  Figure 1 The PCR products of MMP-1-1607 and MMP-1-519 were electrophoresed in 3% agarose gel and stained with 0.1% ethidium bromide. The PCR products were 118 bp and 200 bp for MMP-1-1607 and MMP-1-519, respectively.

• 

  Figure 2 SNPs at MMP-1-1607 and MMP-1-519 after restriction enzyme digestion were electrophoresed in 3% agarose gel and stained with 0.1% ethidium bromide. The allele 1G of MMP-1-1607 gave product of 89 bp and allele 2G gave product of 118 bp. For the MMP-1-519, allele A and allele G gave products of 176 bp and 200 bp respectively.

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