Korean Circ J.  2005 Feb;35(2):192-196. 10.4070/kcj.2005.35.2.192.

Functional Polymorphism in the Promoter Region of Matrix Metalloproteinase-9 is Strongly Associated with Acute Myocardial Infarction

• Kim PJ
• Chang K
• Koh YS
• Seung KB
• Baek SH
• Shin WS
• Lim SH
• Kim JH
• Hong SJ
• Choi KB

• KMID: 2227161
• DOI: http://doi.org/10.4070/kcj.2005.35.2.192

Abstract

BACKGROUND AND OBJECTIVES
Matrix metalloproteinase-9 (MMP-9) plays an important role in the genesis of atherosclerotic plaque rupture and acute coronary syndrome (ACS), including an acute myocardial infarction (AMI). A single nucleotide polymorphism (SNP) in the MMP-9 promoter (-1562C>T) has recently been identified. This study investigated whether the SNP of the MMP-9 promoter is a significant risk factor for an AMI due to plaque rupture and if SNPs affect the transcription of the gene that elevates the MMP-9 expression level.
SUBJECTS AND METHODS
A polymerase chain reaction, followed by a restriction fragment length polymorphism analysis, was performed in 173 control participants and 206 AMI patients. The serum levels of MMP-9 in the groups with or without the SNP were measured, using ELISA, and compared.
RESULTS
There was a significantly higher incidence of the -1562C>T MMP-9 polymorphism in the AMI compared to the control group (27.6% vs. 17.9%, p=0.04). A multiple logistic regression analysis of the risk factors for coronary artery disease and the MMP-9 polymorphism showed the MMP-9 polymorphism to be an important factor in the prediction of an AMI (odds ratio 1.67, 95% confidence interval 1.02-2.67, p=0.04).
CONCLUSION
The -1562C>T polymorphism in the MMP-9 promoter is a definite risk factor for an AMI, and is associated with elevated MMP-9 expression. These results suggest that a SNP in the MMP-9 promoter is strongly associated with an Acute Myocardial Infarction.

Keyword

Matrix metalloproteinase-9; Polymorphism; Acute myocardial infarction

MeSH Terms

Acute Coronary Syndrome
Coronary Artery Disease
Enzyme-Linked Immunosorbent Assay
Humans
Incidence
Logistic Models
Matrix Metalloproteinase 9*
Myocardial Infarction*
Plaque, Atherosclerotic
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide
Promoter Regions, Genetic*
Risk Factors
Rupture
Matrix Metalloproteinase 9