J Korean Acad Periodontol.  2008 Dec;38(4):579-588. 10.5051/jkape.2008.38.4.579.

IL-6 gene promoter polymorphisms in Korean generalized aggressive periodontitis

• Bang SJ
• Kim IS
• Kim OS
• Kim YJ
• Chung HJ

• KMID: 1999589
• DOI: http://doi.org/10.5051/jkape.2008.38.4.579

Abstract

PURPOSE
The purpose of this study was to investigate the association of generalized aggressive periodontitis with IL-6 promoter gene single nucleotide polymorphisms(SNP). MATERIAL AND METHODS: The study population consisted of 52 generalized aggressive periodontitis patients(GAP) and 30 periodontally healthy control subjects, who were systemically healthy non-smokers. Genomic DNA was obtained from buccal swab. The IL-6 promotor SNP at the positions of -597, -572, and -174 were genotyped by amplifying the polymorphic region using polymerase chain reaction(PCR), restriction enzyme digestion and gel electrophoresis. RESULT: The genotype distributions for G/G, G/A and A/A genotypes of IL-6 -597 were 30.8%, 40.4%, and 28.8% in the GAP group and 53.3%, 40%, and 6.7% in the control group and were statistically different between 2 groups(p<0.05). Allele 2 frequency of IL-6 -597 were significantly higher in the GAP group than the control group(p<0.01). At the position of IL-6 -572, the distribution for C/C, C/G and G/G genotypes were 23.1%, 55.8% and 21.2% in the GAP group and 20%, 33.3%, and 46.7% in the control group. In female subjects, the genotype distribution were significantly different between 2 groups (p<0.01). In male subjects, allele 2 frequency of IL-6-572 was significantly lower in the GAP group than the control group(p<0.05). The genotype distribution of IL-6 -174 in the GAP group were 96.2%, 3.8% for G/G, G/C genotypes whereas only the G/G genotype was detected in the control group.
CONCLUSION
In conclusion, significant associations were found in IL-6 gene promoter(-597, -572) polymorphisms and generalized aggressive periodontitis. Further cohort study will be necessary in larger population.

Keyword

aggressive periodontitis; IL-6 gene; SNP; gene polymorphism

MeSH Terms

Aggressive Periodontitis
Alleles
Cohort Studies
Digestion
DNA
Female
Genotype
Humans
Interleukin-6
Male
DNA
Interleukin-6