World J Mens Health.  2013 Aug;31(2):150-156.

The Anti-Inflammatory Effects of a New Herbal Formula (WSY-1075) in a Nonbacterial Prostatitis Rat Model

• Yoon BI
• Bae WJ
• Kim SJ
• Kim HS
• Ha US
• Sohn DW
• Hwang SY
• Kim SW

Abstract

PURPOSE
The aim of this study was to investigate the anti-inflammatory effects of a new herbal formula (WSY-1075) in a nonbacterial prostatitis rat model.
MATERIALS AND METHODS
Prostatitis was induced in male Wistar rats (n=32) by treatment with 17 beta-estradiol and dihydrotestosterone for 4 weeks. After the induction of prostatitis, the rats were randomly divided into one of four treatment groups: control (n=8), ciprofloxacin (n=8), WSY-1075 (100 mg/kg) (n=8), and WSY-1075 (400 mg/kg) (n=8). After 4 weeks of treatment, the prostatic proinflammatory cytokine (tumor necrosis factor-alpha, interleukin [IL]-6, and IL-8) levels and histological findings were noted.
RESULTS
The ciprofloxacin and WSY-1075 treatment groups showed significantly decreased proinflammatory cytokine levels compared with the control group. Histologically, treatment with ciprofloxacin and WSY-1075 significantly suppressed the severity of prostatitis lesions compared with those in the control group. No differences in the proinflammatory cytokine levels or histologic findings were observed with the dose dependent treatment of WSY-1075.
CONCLUSIONS
The new herbal formula, WSY-1075, showed effective anti-inflammatory activities in the prostate and may be useful for the clinical treatment of nonbacterial prostatitis. Our findings suggest that WSY-1075 has a beneficial effect on the prevention and treatment of nonbacterial prostatitis.

Keyword

Herbals; Prostatitis; Inflammation

MeSH Terms

Animals
Ciprofloxacin
Dihydrotestosterone
Estradiol
Humans
Inflammation
Interleukins
Male
Prostate
Prostatitis
Rats
Rats, Wistar
Ciprofloxacin
Dihydrotestosterone
Estradiol
Interleukins

Figure

• 

  Fig. 1 Histopathologic findings of the prostate lateral lobe in each of the 4 groups (H&E, ×100). (A) The control group showed extensive infiltration of inflammatory cells, including neutrophils, lymphocytes, macrophages, and degeneration of glandular epithelial cells. All of the rats showed grade 3 inflammation. (B) The ciprofloxacin group showed a nearly normal appearance of the glandular epithelium with less leukocyte infiltration. All of the rats showed grade 1 inflammation. In the (C) WSY-1075 (100 mg/kg) and (D) WSY-1075 (400 mg/kg) groups, the inflammatory cells in the lumina and the epithelial cells of the gland and stroma showed more improvement than those of the control group but less improvement than those of the ciprofloxacin group. All of the rats showed grade 1~2 inflammation.

• 

  Fig. 2 Effect of WSY-1075 (100 mg/kg) and WSY-1075 (400 mg/kg) on serum tumor necrosis factor-α levels. Each value represents mean±standard deviation. *p<0.05, compared with the control group, †p<0.05, compared with the ciprofloxacin group.

• 

  Fig. 3 Effect of WSY-1075 (100 mg/kg) and WSY-1075 (400 mg/kg) on serum interleukin-6 levels. Each value represents mean±standard deviation. *p<0.05, compared with the control group, †p<0.05, compared with the ciprofloxacin group.

• 

  Fig. 4 Effect of WSY-1075 (100 mg/kg) and WSY-1075 (400 mg/kg) on serum interleukin-8 levels. Each value represents mean±standard deviation. *p<0.05, compared with the control group, †p<0.05, compared with the ciprofloxacin group.

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