World J Mens Health.  2014 Dec;32(3):151-158. 10.5534/wjmh.2014.32.3.151.

Effects of Hydro-Alcoholic Extract of Rhus coriaria (Sumac) Seeds on Reproductive Complications of Nicotinamide-Streptozotocin Induced Type-2 Diabetes in Male Mice

• Ahangarpour A
• Oroojan AA
• Heidari H
• Ehsan G
• Rashidi Nooshabadi MR

• KMID: 2320779
• DOI: http://doi.org/10.5534/wjmh.2014.32.3.151

Abstract

PURPOSE
The purpose of this study was to investigate the effects of the hydro-alcoholic extract of Rhus coriaria seeds on the reproductive system of nicotinamide-streptozotocin-induced type-2 diabetic mice.
MATERIALS AND METHODS
In this experimental study, 56 male Naval Medical Research Institute mice were randomly divided into seven groups (n=8): control; diabetic mice; diabetic mice administered glibenclamide (0.25 mg/kg); diabetic mice who received the hydro-alcoholic extract of R. coriaria seeds (200 and 400 mg/kg groups); and normal mice who received this extract (200 and 400 mg/kg groups). Diabetes was induced by intraperitoneal administration of streptozotocin (65 mg/kg) 15 minutes after an injection of nicotinamide (120 mg/kg). Then, glibenclamide and the above mentioned extract were administered orally for 28 consecutive days. Twenty-four hours after the last treatment, serum samples, the testes, and the cauda epididymis were removed immediately for hormonal, testis morphology, and sperm parameter assessments.
RESULTS
Body and testicular weight, sperm count and viability, and serum luteinizing hormone, follicle-stimulating hormone and testosterone levels were significantly lower in the diabetic mice (p<0.05). The diabetic mice treated with 400 mg/kg of the hydro-alcoholic extract of R. coriaria seeds recovered from these reductions (p<0.05). Further, glibenclamide alleviated hormonal and sperm count depletion in diabetes-induced mice (p<0.05).
CONCLUSIONS
The present results indicated that the hydro-alcoholic extract of R. coriaria seeds has anti-infertility effects in diabetic males.

Keyword

Diabetes mellitus; Nicotinamide; Spermatogenesis; Antioxidant effect; Streptozotocin

MeSH Terms

Academies and Institutes
Animals
Antioxidants
Diabetes Mellitus
Epididymis
Follicle Stimulating Hormone
Glyburide
Humans
Luteinizing Hormone
Male
Mice*
Niacinamide
Rhus*
Sperm Count
Spermatogenesis
Spermatozoa
Streptozocin
Testis
Testosterone
Antioxidants
Follicle Stimulating Hormone
Glyburide
Luteinizing Hormone
Niacinamide
Streptozocin
Testosterone

Figure

• 

  Fig. 1 Effect of hydro-alcoholic extract of Rhus coriaria (S) seeds on sperm count in normal and nicotinamide-streptozotocin-induced diabetic mice. Values are presented as mean±standard error; n=8. GLI: glibenclamide, S: Sumac. ap<0.05 compared with the diabetic group, bp<0.05 compared with the control group.

• 

  Fig. 2 Effect of hydro-alcoholic extract of Rhus coriaria (S) seeds on sperm viability in normal and nicotinamide-streptozotocin-induced diabetic mice. Values are presented as mean±standard error; n=8. GLI: glibenclamide, S: Sumac. ap<0.05 compared with the diabetic group, bp<0.05 compared with the control group.

• 

  Fig. 3 Effect of hydro-alcoholic extract of Rhus coriaria (S) seeds on serum luteinizing hormone (LH) levels in normal and nicotinamide-streptozotocin-induced diabetic mice. Values are presented as mean±standard error; n=8. GLI: glibenclamide, S: Sumac. ap<0.05 compared with the diabetic group, bp<0.05 compared with the control group.

• 

  Fig. 4 Effect of hydro-alcoholic extract of Rhus coriaria (S) seeds on serum follicle-stimulating hormone (FSH) levels in normal and nicotinamide-streptozotocin-induced diabetic mice. Values are presented as mean±standard error; n=8. GLI: glibenclamide, S: Sumac. ap<0.05 compared with the diabetic group, bp<0.05 compared with the control group.

• 

  Fig. 5 Effect of hydro-alcoholic extract of Rhus coriaria (S) seeds on serum testosterone level in normal and nicotinamide-streptozotocin-induced diabetic mice. Values are presented as mean±standard error; n=8. GLI: glibenclamide, S: Sumac. ap <0.05 compared with the diabetic group, bp<0.05 compared with the control group.

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