World J Mens Health.
2021 Jan;39(1):147-157. 10.5534/wjmh.190010.
Effects of Myricitrin and Solid Lipid NanoparticleContaining Myricitrin on Reproductive System Disorders Induced by Diabetes in Male Mouse
- Affiliations
-
- 1Department of Pharmacology and Toxicology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran.
- 2Health Research Institute, Diabetes Research Center, Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
- 3Department of Physiology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran.
- KMID: 2509591
- DOI: http://doi.org/10.5534/wjmh.190010
Abstract
- Purpose
The present study investigates the effects of myricitrin and solid lipid nanoparticle (SLN) containing myricitrin on the reproductive system of type 2 diabetic male mice.
Materials and Methods
In this experimental study, SLN containing myricitrin was prepared by the cold homogenization method. Then, 90 adult male Naval Medical Research Institute mice were divided into 9 groups (n=10): control, vehicle, diabetic, diabetic+myricitrin or SLN containing myricitrin 1, 3, and 10 mg/kg. Diabetes was induced by streptozotocin (65 mg/kg) 15 minutes after nicotinamide (120 mg/kg) injection. Myricitrin and SLN containing myricitrin administered during 1 month. At the 34th days of the experiment, plasma and tissue samples were taken for experimental assessments.
Results
Testis weight and volume decreased in the diabetic group. These variables increased in diabetic treated mice by a high dose of myricitrin or all doses of SLN containing myricitrin (p<0.05). Total antioxidant capacity and superoxide dismutase levels decreased in diabetic mice, and administration of myricitrin 10 mg/kg or all doses of SLN containing myricitrin increased them (p<0.05). Luteinizing hormone, Follicle-stimulating hormone, testosterone, and sperm count decreased in the diabetic group, treatment with a high dose of myricitrin or all doses of SLN containing myricitrin recovered them (p<0.05). Diabetes induced vacuoles and apoptosis in testicular cells, meanwhile myricitrin and SLN containing myricitrin improved them (p<0.05).
Conclusions
Diabetes induced reproductive problem via increased oxidative stress and decrease antioxidant capacity, administration of myricitrin or SLN containing myricitrin improved them. Further, SLN containing myricitrin was more potent than myricitrin.
