Tuberc Respir Dis.  2012 Feb;72(2):156-162.

MAGE Gene Expression in Bronchial Washing Fluid in Suspected Parenchymal Lung Cancer

• Kim KJ
• Choi EY
• Shin KC

Abstract

BACKGROUND
The main goal of this study was to evaluate the diagnostic efficacy of reverse transcription-nested polymerase chain reaction (RT-nested PCR) in bronchial washing fluid with MAGE A1-6 common primers for the detection of lung cancers invisible by bronchoscopy.
METHODS
To determine the expression of MAGE A1-6 gene in 189 lung cancers diagnosed by conventional fluoroscopy-guided lung biopsy and 89 cancer-free controls, RT-nested PCR was performed in bronchial washing specimens. We analyzed MAGE A1-6 RT-nested PCR data according to tumor histology, stage, size, and compared them with cytological data.
RESULTS
189 patients (111 cases in adenocarcinoma, 47 cases in squamous cell carcinoma, 22 cases in small cell lung carcinoma, and 9 cases in other cancers) and 89 benign patients were investigated. The expression of MAGE was performed by nested RT-PCR using common MAGE primer. Among 189 cancer patients, the expression rate of MAGE was 49.2%, and the positive predictive value was 89.4%. However, the expression rate of MAGE in patients with benign lesions was 12.4%. In peripheral lung cancer, the positive rate of MAGE expression was 57.4% in squamous cell carcinoma, 44.1% in adenocarcinoma and 59.1% in small cell lung cancer. Whereas the expression rate of bronchial washing cytology in peripheral lung cancer was 9.0% (p=0.011).
CONCLUSION
MAGE RT-PCR in bronchial washing fluid gave us promising data for the detection of peripheral lung cancer. It could be a useful method for selecting diagnostic tools for peripheral lesions.

Keyword

Lung Neoplasms; Early Detection of Cancer; Melanoma-Specific Antigens; Reverse Transcriptase Polymerase Chain Reaction

MeSH Terms

Adenocarcinoma
Biopsy
Carcinoma, Squamous Cell
Early Detection of Cancer
Gene Expression
Humans
Lung
Lung Neoplasms
Melanoma-Specific Antigens
Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Small Cell Lung Carcinoma
Melanoma-Specific Antigens

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