Tuberc Respir Dis.  2006 Mar;60(3):330-336.

Aquaporin in bleomycin induced lung injury

Affiliations
  • 1Division of Allergy and Respintoy Diseases, Depotmet of Intenal Medicine Soonchunhyang University Hospital, Korea. mdcspark@unitel.co.kr

Abstract

BACKGROUND: Aquaporins (AQPs) may play a role in the pathogenesis of pulmonary inflammation and edema. This study investigated the role ofAQPs in acute lung injury following bleomycin inhalation in rats.
METHODS
Sprague-Dawley rats were treated via inhalation with 10 U/kg bleomycin hydrochloride dissolved in 5 ml of normal saline. The control rats were treated with 5 ml normal saline. The animals (n = 6-8 rats per group) were sacrificed at 4, 7, and 14 d. The changes in AQP1, AQP4, and AQP5 expression levels over time were analyzed by Western blotting. The nitrate and nitrite concentrations in the bronchoalveolar lavage fluid (BALF) were measured using a modified Griess reaction. ELISA was used to check cytokines.
RESULTS
The respiration rates were significantly higher 4 and 7 days after the bleomycin treatment compared with those of the control rats. The tidal volume was lower in rats at 4 days after the bleomycin treatment, and the wet/dry weights of the lung were significantly higher than those of the control group. The nitrite and nitrate concentrations in the BALF from the rats at 4 days after exposure to bleomycin were greater than those from the saline-treated rats. Immunoblotting studies demonstrated that the AQP1 and AQP4 expression levels were lower in the rats at 4 days. However, the AQP4 expression level was higher at 7 days. The AQP5 expression level increased at 4, 7 and 14 days after the bleomycin treatment.
CONCLUSION
This study demonstrates that AQPs are expressed differently in bleomycin-induced pulmonary edema.

Keyword

Aquaporin; Nitric oxides; acute lung injury

MeSH Terms

Acute Lung Injury
Animals
Aquaporins
Bleomycin*
Blotting, Western
Bronchoalveolar Lavage Fluid
Cytokines
Edema
Enzyme-Linked Immunosorbent Assay
Immunoblotting
Inhalation
Lung Injury*
Lung*
Pneumonia
Pulmonary Edema
Rats
Rats, Sprague-Dawley
Respiratory Rate
Tidal Volume
Weights and Measures
Aquaporins
Bleomycin
Cytokines

Figure

  • Figure 1 Increased lung fluid after bleomycin treatment. *p<0.05 compared with saline treatment, †p<0.01 compared with 4 days after bleomycin treatment.

  • Figure 2 Pulmonary infiltrates were observed in the peribronchial and perivascular regions with H& E stains. Original magnification, ×100. Alveolar interstitial edema was detected as thickened alveolar septa in some regions. Evidence of pulmonary edema was generally more evident in the lungs of rats at 4 days after bleomycin treatment (B) than in rats treated with saline alone (A).

  • Figure 3 Increased nitrite and nitrate levels after bleomycin treatment. *p<0.01 compared with saline treatment, †p<0.01 compared with 4 days (d) after bleomycin treatment.

  • Figure 4 Lung aquaporin 1 (AQP1), AQP4 and AQP5 protein expression after bleomycin treatment. *p<0.05 compared with saline treatment, †p<0.01 compared with saline treatment.


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