Psychiatry Investig.  2015 Jan;12(1):81-91. 10.4306/pi.2015.12.1.81.

Profiling of Proteins Regulated by Venlafaxine during Neural Differentiation of Human Cells

• Doh MS
• Han DM
• Oh DH
• Kim SH
• Choi MR
• Chai YG

• KMID: 2316393
• DOI: http://doi.org/10.4306/pi.2015.12.1.81

Abstract

OBJECTIVE
Antidepressants are known to positively influence several factors in patients with depressive disorders, resulting in increased neurogenesis and subsequent relief of depressive disorders. To study the effects of venlafaxine during neural differentiation at the cellular level, we looked at its effect on protein expression and regulation mechanisms during neural differentiation.
METHODS
After exposing NCCIT cell-derived EBs to venlafaxine during differentiation (1 day and 7 days), changes in protein expression were analyzed by 2-DE and MALDI-TOF MS analysis. Gene levels of proteins regulated by venlafaxine were analyzed by real-time RT-PCR.
RESULTS
Treatment with venlafaxine decreased expression of prolyl 4-hydroxylase (P4HB), ubiquitin-conjugating enzyme E2K (HIP2) and plastin 3 (T-plastin), and up-regulated expression of growth factor beta-3 (TGF-beta3), dihydropyrimidinase-like 3 (DPYSL3), and pyruvate kinase (PKM) after differentiation for 1 and 7 days. In cells exposed to venlafaxine, the mRNA expression patterns of HIP2 and PKM, which function as negative and positive regulators of differentiation and neuronal survival, respectively, were consistent with the observed changes in protein expression.
CONCLUSION
Our findings may contribute to improve understanding of molecular mechanism of venlafaxine.

Keyword

Venlafaxine; Depression; NCCIT cells; Proteomics; HIP2; Pyruvate kinase

MeSH Terms

Antidepressive Agents
Depression
Depressive Disorder
Humans
Neurogenesis
Neurons
Prolyl Hydroxylases
Proteomics
Pyruvate Kinase
RNA, Messenger
Venlafaxine Hydrochloride
Antidepressive Agents
Pyruvate Kinase
RNA, Messenger