Osteoporosis.  2011 Apr;9(1):66-79.

The Relationship between Polymorphisms in Tumor Necrosis Factor (TNF) Genes and Changes in Bone Mineral Density after Hormone Therapy in Postmenopausal Korean Women

• Chun S
• Kim JG

Abstract

OBJECTIVES
To determine the association between polymorphisms in tumor necrosis factor-alpha (TNF-alpha), and TNF-beta genes, and changes in bone mineral density (BMD) after hormone therapy (HT) in postmenopausal Korean women. MATERIALS & METHODS: The TNF-alpha G(-308)A, C(-857)T, C(-863)A, T(-1031)C, and TNF-beta A252G polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) or Taqman assay in 218 postmenopausal Korean women receiving sequential HT for 1 year. Bone mineral density (BMD) at the lumbar spine and femoral neck was examined by dual energy X-ray absorptiometry. Serum levels of bone specific alkaline phosphatase (BAP), osteocalcin (OST), type I C-telopeptide breakdown products (CTX), parathyroid hormone (PTH), calcium, and phosphorus were measured using enzyme-linked immunosorbent assay (ELISA), immunoassay, and atomic absorptiometry, respectively.
RESULTS
The TNF-alpha G(-308)A, C(-857)T, C(-863)A, T(-1031)C, and TNF-beta A252G polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) or Taqman assay in 218 postmenopausal Korean women receiving sequential HT for 1 year. Bone mineral density (BMD) at the lumbar spine and femoral neck was examined by dual energy X-ray absorptiometry. Serum levels of bone specific alkaline phosphatase (BAP), osteocalcin (OST), type I C-telopeptide breakdown products (CTX), parathyroid hormone (PTH), calcium, and phosphorus were measured using enzyme-linked immunosorbent assay (ELISA), immunoassay, and atomic absorptiometry, respectively
CONCLUSIONS
The TNF-alpha G(-308)A, C(-857)T, C(-863)A, T(-1031)C, and TNF-beta A252G polymorphisms are not associated with changes in BMD after HT in postmenopausal Korean women.

Keyword

Korean postmenopausal women; TNF; Gene polymorphism; Hormone therapy; BMD

MeSH Terms

Absorptiometry, Photon
Alkaline Phosphatase
Bone Density
Calcium
Collagen Type I
Enzyme-Linked Immunosorbent Assay
Female
Femur Neck
Humans
Immunoassay
Lymphotoxin-alpha
Osteocalcin
Parathyroid Hormone
Peptides
Phosphorus
Spine
Tumor Necrosis Factor-alpha
Alkaline Phosphatase
Calcium
Collagen Type I
Lymphotoxin-alpha
Osteocalcin
Parathyroid Hormone
Peptides
Phosphorus
Tumor Necrosis Factor-alpha