Mycobiology.
2010 Mar;38(1):74-77.
Development of Detection Methods for Cellulolytic Activity of Auricularia auricula-judae
- Affiliations
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- 1Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration, Daegu 702-320, Korea. jowoosik@yahoo.co.kr
- 2Mushroom Research Division, Department of Herbal Crop Research, National Institute of Horicultural and Herbal Science, RDA, Suwon 404-707, Korea.
- 3College of Veterinary Medicine, Kyeongbuk National University, Daegu 702-701, Korea.
Abstract
- To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent, pH, and temperature were assessed. Chromogenic dye (congo red, phenol red, remazol brilliant blue, and trypan blue) was individually incorporated into a medium containing either carboxymethyl-cellulose, Avicel, or D-cellobiose as a polysaccharide carbon substrate. The other assessments utilized pHs ranging from 4.5 to 8.0 and temperatures from 15~35degrees C. Overall, when A. auricula-judae species were transferred onto media contained Congo red and adjusted pH 7.0 and then incubated at 25degrees C for 5 days, the clear zone indicative of cellulolytic activity was more pronounced.
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