Mycobiology.  2018 Mar;46(1):72-78. 10.1080/12298093.2018.1454004.

Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae

Affiliations
  • 1College of Horticulture, Jilin Agricultural University, Changchun, Jilin Province, PR China. yaofj@aliyun.com
  • 2Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin Province, PR China.
  • 3Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan.

Abstract

The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189-522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker "SCL-18" consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.

Keyword

Auricularia auricula-judae; fruiting body pattern; sequence-related amplified polymorphism; sequence characterized amplified region

MeSH Terms

Breeding
Chrysanthemum
Cicatrix
Clone Cells
Fruit*
Fungi
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