Mycobiology.  2009 Dec;37(4):313-316. 10.4489/MYCO.2009.37.4.313.

Optimal Medium Conditions for the Detection of Cellulolytic Activity in Ganoderma lucidum

• Jo WS
• Bae SH
• Cho DH
• Park SD
• Yoo YB
• Park SC

• KMID: 2053036
• DOI: http://doi.org/10.4489/MYCO.2009.37.4.313

Abstract

To determine the optimal medium conditions for the detection of the cellulolytic activity in Ganoderma lucidum, we varied three media conditions: dye reagent, pH, and temperature. First, we evaluated the use of four dyes, Congo Red, Phenol Red, Remazol Brilliant Blue, and Trypan Blue. To observe the effect of pH on the chromogenic reaction, we also made and tested various media spanning acidic and alkaline pHs, ranging from 4.5 to 8.0. Furthermore, in order to research the effect of temperature on the clear zone and the fungus growing zone, we tested temperatures ranging from 15 to 35degrees C. On the whole, the best protocol called for Ganoderma lucidum transfer onto media containing Congo red with pH adjusted to 7.0, followed by incubation at 25degrees C for 5 days. Our results will be useful to researchers who aim to study extracellular enzyme activity in Ganoderma lucidum.

Keyword

Cellulolytic enzyme; Chromogenic media; Congo red; Ganoderma lucidum

MeSH Terms

Benzenesulfonates
Coloring Agents
Congo Red
Diminazene
Fungi
Ganoderma
Hydrogen-Ion Concentration
Phenolsulfonphthalein
Reishi
Trypan Blue
Benzenesulfonates
Coloring Agents
Congo Red
Diminazene
Phenolsulfonphthalein
Trypan Blue