Lab Anim Res.  2010 Sep;26(3):257-263. 10.5625/lar.2010.26.3.257.

Antibacterial Activity of Sanguisorba officinalis against Helicobacter pylori

• Lee HA
• Hong S
• Oh HG
• Park SH
• Kim YC
• Park H
• Jeong GS
• Kim O

• KMID: 2312075
• DOI: http://doi.org/10.5625/lar.2010.26.3.257

Abstract

In this study, a medicinal herbal plant, Sanguisorba officinalis, was examined and screened for anti-Helicobacter pylori (H. pylori) activity. Seventy percent ethanol was used for herbal extraction. For anti-H. pylori activity screening, inhibitory zone tests as an in vitro assay and in vivo study using a Mongolian gerbil (Meriones unguiculatus) model were performed. Also, the safety of herbal compounds was evaluated by animal study. As a result of inhibitory zone test, Sanguisorba officinalis extract demonstrated strong anti-H. pylori activities. Also, as results of in vivo animal studies, Sanguisorba officinalis extract demonstrated strong therapeutic effects against H. pylori infection according to the criteria of histological examination and rapid urease test. As results of the safety study, after 28 days treatment of the Sanguisorba officinalis extract, the animals were not detected any grossly and histological changes. These results demonstrate that it can be successfully cured against H. pylori infection and protected from H. pylori-induced pathology with Sanguisorba officinalis extract. It could be a promising candidate herb treatment for patients with gastric complaints including gastric ulcer caused by H. pylori.

Keyword

Sanguisorba officinalis; Helicobacter pylori; antibacterial activity; herb; therapy

MeSH Terms

Animals
Ethanol
Gerbillinae
Helicobacter
Helicobacter pylori
Humans
Mass Screening
Plants
Plants, Medicinal
Sanguisorba
Stomach Ulcer
Urease
Ethanol
Urease

Figure

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  Figure 1. Results of polymerase chin reaction in the study of anti-H. pylori activity with Sanguisorba officinalis extract. M: 100 bp marker, P: DNA from H. pylori ATCC 43504, N: negative control, 1~2: Group I (H. pylori+Sanguisorba officinalis), 3~6: Group II (H. pylori + phosphate-buffered saline).

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