Korean J Blood Transfus.
2006 Jun;17(1):61-70.
Serologic Variability of the A(var) (784G>A) and Its Property of Different Expression Depending on Co-inherited ABO Allele
- Affiliations
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- 1Department of Laboratory Medicine, Chonnam National University School of Medicine, Gwangju, Korea. hasomii@hanmail.net
- 2Gwangju-Chonnam Cross Blood Center, Korean Red Cross, Namwon, Korea.
- 3Blood Transfusion Research Institute, Seoul, Korea.
- 4Department of Laboratory Medicine, Seonam University School of Medicine, Seoul, Korea.
Abstract
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BACKGROUND: A allele, A(var), characterized by a 784G>A polymorphism (Asp262Asn) has been identified only in Korean A(weak)B donors. This study evaluated the serological and genetic characteristics of thirteen samples with newly identified A(var) allele.
METHODS
This study examined 10 samples with the A(var) allele including 4 members from a family, who were randomly obtained from blood donors recruited at Gwangju-Chonnam Red Cross Blood Center, and patients at the Chonnam National University Hospital. Routine ABO serologic tests, ABO genotyping using an allele specific polymerase chain reaction (AS-PCR), and the sequencing of exon 6 and 7 of ABO gene were performed on all samples. In addition, sequencing of exon 1~5 of the ABO gene was carried out on two randomly selected samples.
RESULTS
The A(var) allele was identified in nine A(weak)B and one O (II-1 of the family study) sample. Eight of these nine individuals showed 1+ agglutination with the monoclonal anti-A reagents on forward typing but one sample showed no agglutination. Weak anti-A was detected in all sera. From the family study, the A(var) allele, which was transmitted from the propositus through her descendant (II-1, II-3 and III-1), produced either the weak A phenotype when inherited with a B allele or the O phenotype when inherited with an O allele.
CONCLUSION
A(var) erythrocytes showed different agglutination patterns to anti-A. Different expressions (possible allelic enhancement) were observed depending on the co-inherited ABO alleles from samples with the A(var) allele.
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