Korean J Asthma Allergy Clin Immunol.  2005 Dec;25(4):305-312.

The Effects of Corticosteroids on IL-6, IL-8 and RANTES Production from RSV-infected Epithelial Cells

Abstract

BACKGROUND
RSV induces the production of cytokines and chemokines in airway epithelial cells. Corticosteroids (CSs) can inhibit the production.
OBJECTIVE
We examined whether the inhibitory effect of CSs on cytokines and chemokines production in airway epithelial cells infected with RSV varies according to both the kinds of CSs and and to the timing of CSs treatment. METHOD: As the CSs pretreatment group, BEAS-2B cells were pretreated with budesonide (BUD), fluticasone propionate (FP), or methylprednisolone (MP), and then the cells were infected with RSV. As the CSs post-treatment group, BEAS-2B cells were infected with RSV. The cells were next treated with CSs. Interleukin (IL)-6, IL-8, and RANTES (regulated on activation, normal T cell expressed and secreted) levels in each of the collected supernatan were determined. RESULT: In the CSs pretreatment group, the strength of the inhibitory effect was in the order of MP>FP>BUD on IL-6 production. Regarding the inhibitory effect on IL-8 or RANTES production, no significant difference was detected. In the CSs post-treatment group, the strength of the inhibitory effect was in the order of MP>BUD>FP on the production of IL-6. MP=FP >BUD on IL-8 production. BUD>FP=MP on RANTES production. CONCLUSION: The inhibitory effects of CSs on the production of IL-6, IL-8, and RANTES in BEAS-2B cells infected with RSV varies widely according to the kinds of cytokines, the kinds of CSs and the timing of the CSs treatment.

MeSH Terms

Adrenal Cortex Hormones*
Budesonide
Chemokine CCL5*
Chemokines
Cytokines
Diethylpropion
Epithelial Cells*
Interleukin-6*
Interleukin-8*
Interleukins
Methylprednisolone
Respiratory Syncytial Viruses
Fluticasone
Adrenal Cortex Hormones
Budesonide
Chemokine CCL5
Chemokines
Cytokines
Diethylpropion
Interleukin-6
Interleukin-8
Interleukins
Methylprednisolone