Korean J Urol.  2003 May;44(5):446-451.

Correlation between Up-Regulation of Caveolin-1 Gene in Human Rencal Cell Carcinoma and Multi-drug Resistance

• Shim HY
• Choi HY

Abstract

PURPOSE: To investigate whether up-regulation of the caveolin-1 gene is associated with the resistance to the chemotherapeutic agents in human renal cell carcinomas (HRCC).
MATERIALS AND METHODS
Two HRCC cell lines, SN12C and SN12CPM7, with low and high metastatic potentials, respectively, were cultured. Between these two cell lines, the cytotoxicities to doxorubicin, and the expressions of the caveolin-1 gene, were compared using the MTT assay and Northern blot analysis, respectively. A full length of caveolin-1 cDNA was obtained, and inserted into the SN12C to make stable cells expressing the caveolin-1 gene (SN12C/Cav). In these cells, the status of the caveolin-1 expression was evaluated, and the cytotoxicity to doxorubicin or interferon compared to other cell lines.
RESULTS
The SN12CPM7 cells were less sensitive to the doxorubicin than the SN12C cells in the cytotoxicity study. The Northern blot analysis revealed that the expression of caveolin-1 was higher in the SN12CPM7 than in the SN12C cells. The expression levels of the caveolin-1 gene in the SN12C/Cav and SN12CPM7 were very similar. The MTT assay, using transfected cell lines, revealed that the SN12C/Cav was more resistant to both the doxorubicin and interferon than the SN12C.
CONCLUSIONS
In HRCCs, up-regulation of the caveolin-1 gene may be associated with the change in the biological response to anti-cancer drugs. The induction of the caveolin-1 gene expression may provide the cancer cells with some protection against the action of cytotoxic drugs.

Keyword

Carcinoma; renal cell; Human; Caveolin-1; Caveolins; Drug resistance; multiple

MeSH Terms

Blotting, Northern
Carcinoma, Renal Cell
Caveolin 1*
Caveolins
Cell Line
DNA, Complementary
Doxorubicin
Drug Resistance
Drug Resistance, Multiple*
Gene Expression
Humans*
Interferons
Up-Regulation*
Caveolin 1
Caveolins
DNA, Complementary
Doxorubicin
Interferons