Korean J Urol.  1991 Aug;32(4):519-529.

Histochemical and ultrastructural changes of cyclosporin A and prednisolone on proximal tubules and glomeruli in rabbit kidney

• Chang SG
• Bae GW

Abstract

This experiment was designed to investigate the histochemical and ultrastructural changes of the proximal tubules and glomeruli of rabbits treated with cyclosporin A (CsA) or cyclosporin A and concomitant administration of prednisolone (PD). Rabbits were given with CsA for 1 week (CsAlW) and for 2 weeks (CsA2W) and CsA plus PD for 1 week (CsA +PDIW) and for 2 weeks (CsA+PD2W). The dose of CsA was 5mg/kg/day i.v. and PD was 1 mg/kg/day i.m. At 1 week and 2 weeks after the treatments, the following items were studied of alkaline phosphatase (ALPase). peroxidase, 5'-nucleotidase (SNU) with the ultrastructural enzymatic changes and electron microscopic changes. CsAlW, CsA2W and CsA +PD2W groups showed a decrease of ALPase activity and CsA+PD2W and CsA2W groups showed weakly increased peroxidase activity CsA2W and CsA+PD2W showed a decrease of 5NU activity. Ultrastructural changes of CsAlW CsA2W and CsA+PD2W groups revealed swollen and disrupted mitochondria, loss of brush border in the proximal tubules and thickening of basement membranes, edematous podocytes and fusion of root processes of podocytes in the glomeruli showing the early stage of nephrotoxicity. The concomitant treatment of PD with CsA eased the nephrotoxicity of CsA within 1 week after the treatment.

Keyword

Cyclosporin A; Prednisolone; Nephrotoxicity

MeSH Terms

5'-Nucleotidase
Alkaline Phosphatase
Basement Membrane
Cyclosporine*
Kidney*
Microvilli
Mitochondria
Peroxidase
Podocytes
Prednisolone*
Rabbits
5'-Nucleotidase
Alkaline Phosphatase
Cyclosporine
Peroxidase
Prednisolone