Korean J Reprod Med.  2007 Dec;34(4):275-283.

Expression of Endometriosis Related Genes in the Shed Endometrial Tissues from Menstrual Blood

• Park CW
• Jun JH
• Koong MK
• Song IO

Abstract

OBJECTIVE
This study was performed to investigate the expressions of endometriosis related genes in shed endometrial tissues from menstrual blood of patients with or without endometriosis.
METHODS
The shed endometrial tissues were collected on 2nd or 3rd day of menstrual cycle with Wallace catheter in patients with endometriosis (n=16) and without endometriosis (n=26). The mRNA expressions of twelve kinds of endometriosis related genes were compared between two groups using semi-quantitative RT-PCR.
RESULTS
The collected shed endometrium was confirmed by histological observation. Expressions of telomerase, c-kit and aromatase mRNA were not detected by RT-PCR in shed endometrial tissues. The mRNA expressions of apoptosis related genes (fas, fas ligand, bcl-2, bax), stem cell factor, estrogen receptor-alpha/alpha, endometriosis protein-I and secretory leukocyte protease inhibitor gene were similar between shed endometrial tissues with endometriosis and without endometriosis.
CONCLUSION
We could not find the difference of mRNA expressions of tested endometriosis related genes between shed endometrial tissues with or without endometriosis by semi-quantitative RT-PCR analysis. It may be related to the dynamical changes of gene expressions in the endometrium with menstrual cycle.

Keyword

Endometriosis; Shed endometrial tissues; mRNA expression; Semi-quantitative RT-PCR

MeSH Terms

Apoptosis
Aromatase
Catheters
Endometriosis*
Endometrium
Estrogens
Fas Ligand Protein
Female
Gene Expression
Humans
Menstrual Cycle
RNA, Messenger
Secretory Leukocyte Peptidase Inhibitor
Stem Cell Factor
Telomerase
Aromatase
Estrogens
Fas Ligand Protein
RNA, Messenger
Secretory Leukocyte Peptidase Inhibitor
Stem Cell Factor
Telomerase