J Breast Cancer.  2011 Jun;14(2):96-103. 10.4048/jbc.2011.14.2.96.

Expression of Leptin, Leptin Receptor, Adiponectin, and Adiponectin Receptor in Ductal Carcinoma In Situ and Invasive Breast Cancer

Affiliations
  • 1Department of Surgery, Catholic University of Daegu School of Medicine, Daegu, Korea.
  • 2Department of Molecular Biology, Kyung Hee University College of Pharmacy, Seoul, Korea.
  • 3Department of Pathology, Catholic University of Daegu School of Medicine, Daegu, Korea. ap510@cu.ac.kr

Abstract

PURPOSE
Adipocytokines, such as leptin, resistin, and adiponectin, are associated with obesity and breast cancer. Several studies have indicated that adipocytokines may influence tumor growth or differentiation. The aims of this study were to determine the expression of leptin, leptin receptor (ObR), adiponectin and adiponectin receptor (AdipoR) in human breast cancer, to evaluate their prognostic significance in the breast cancer.
METHODS
Specimens from 198 patients with primary breast cancer were enrolled, and representative paraffin tumor blocks were selected for constructing tissue microarrarys (TMA). Immunohistochemical staining for leptin, ObR, adiponectin, and AdipoR was performed using TMA, and the clinicopathologic characteristics were evaluated from the patient's medical records.
RESULTS
Stage 0 breast cancer accounted for 41 cases, and 157 cases were invasive cancer. Positive rates of leptin and ObR expression in the ductal carcinoma in situ (DCIS) group were significantly higher than those of the invasive cancer group (97.4% vs. 34.0%, p<0.001; 74.4% vs. 29.8%, p<0.001). However, positive rates of adiponectin and AdipoR expression in the invasive cancer group were significantly higher than those in the DCIS group (53.7% vs. 33.3%, p=0.024; 59.9% vs. 26.3%, p<0.001). High leptin expression was significantly associated with high Ki-67 expression (p=0.016). High adiponectin expression was significantly correlated with smaller tumor size (p=0.001).
CONCLUSION
We suggest that losses of leptin and ObR expression could be associated with invasive cancer, whereas high adiponectin and AdipoR expression may be associated with breast cancer invasiveness.

Keyword

Adipocytokine; Adiponectin; Breast neoplasms; Leptin

MeSH Terms

Adipokines
Adiponectin
Breast
Breast Neoplasms
Carcinoma, Ductal
Carcinoma, Intraductal, Noninfiltrating
Humans
Leptin
Obesity
Paraffin
Receptors, Adiponectin
Receptors, Leptin
Resistin
Adipokines
Adiponectin
Leptin
Paraffin
Receptors, Adiponectin
Receptors, Leptin
Resistin

Figure

  • Figure 1 Representative examples of immunohistochemical expression of leptin (A, E), leptin receptor (B, F), adiponectin (C, G), and adiponectin receptor (D, H) in invasive ductal carcinoma (upper row) and ductal carcinoma in situ (lower row). Staining for leptin, leptin receptor, adiponectin, and adiponectin receptor appears in the cytoplasm of tumor cells. We interpreted the results semi-quantitatively based on the number of stained cells and signal intensity (immunohistochemical staining, ×200). Scale bar means 200 micrometer.

  • Figure 2 Comparison of leptin (A), leptin receptor (obR) (B), adiponectin (C), and adiponectin receptor (AdipoR) (D) expression levels between patients with ductal carcinoma in situ (DCIS) and invasive breast cancer. Expression of leptin and ObR were significantly higher in the DCIS group than in the invasive cancer group, and adiponectin and AdipoR expression was significantly higher in the invasive cancer group than the DCIS group.


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