J Asthma Allergy Clin Immunol.  1999 Dec;19(6):935-941.

Exposure to toluene diisocyanate ( TDI) induces IL - 8 and RANTES production from bronchial epithelial cell

Abstract

BACKGROUND: There have been a few reports suggesting involvement of neutrophil as well as eosinophil in inducing bronchoconstriction aft,er inhalation of TDI.
OBJECTIVE
In order to observe the source of chemokines in TDI-induced asthma, this investigation was designed to determine whether IL-8 and RANTES could be produced by human bronchial epithelial cells and whether dexamethasone had any effects on their production. Materials and
METHODS
We cultured Beas-2B, a bronchial epithelial cell line, with five concentrations of TDI-human serum albumin (HSA) conjugate and compared them with those having no conjugate. The levels of IL-8 and RANTES in the supernatant were measured by ELISA. To evaluate the effect of pro-inflammatory cytokines, cells were incubated with peripheral blood mononuclear cell (PBMC) culture supernatant, which was derived from PBMC culture of a TDI -induced asthmatic subject under exposure to TDI-HSA conjugate, and then compared to those without PBMC supernatant addition. To evaluate the effect of dexamethasone, four concentrations of dexamethasone were pre-incubated and the same steps were repeated.
RESULTS
There was significant production of IL-8 from bronchial epithelial cells with addition of TDI-HSA conjugate in a dose-dependent manner (p<0.05, respectively), which was significantly augmented with additions of PBMC supernatant (p<0.05, respectively) at each concentration. RANTES production was negligible, however, it increased significantly with addition of PBMC supernatant and TDI-HSA conjugate in a dose response manner(p<0.05, respectively). Compared to the untreated sample, pre-treatment of dexamethasone induced remarkable inhibitions of IL-8 and RANTES production.
CONCLUSION
These results suggest that IL-8 and RANTES released from bronchial epithelial cells may contribute to neutrophil and eosinophil recruitment occurring in TDI-induced airway.

Keyword

TDI; epithelial cell; IL-8; RANTES

MeSH Terms

Asthma
Bronchoconstriction
Chemokine CCL5*
Chemokines
Cytokines
Dexamethasone
Enzyme-Linked Immunosorbent Assay
Eosinophils
Epithelial Cells*
Humans
Inhalation
Interleukin-8
Neutrophils
Serum Albumin
Toluene 2,4-Diisocyanate*
Toluene*
Chemokine CCL5
Chemokines
Cytokines
Dexamethasone
Interleukin-8
Serum Albumin
Toluene
Toluene 2,4-Diisocyanate
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