J Korean Med Sci.  2003 Dec;18(6):809-812. 10.3346/jkms.2003.18.6.809.

Exposure to Toluene Diisocyanate (TDI) Induces IL-8 Production from Bronchial Epithelial Cells: Effect of Pro-inflammatory Cytokines

• Lee YM
• Kim HA
• Park HS
• Lee SK
• Nahm DH

• KMID: 1086736
• DOI: http://doi.org/10.3346/jkms.2003.18.6.809

Abstract

This investigation was designed to confirm IL-8 production from human bronchial epithelial cells with toluene diisocyanate (TDI) exposure and to examine the effects of pro-inflammatory cytokine and dexamethasone. We cultured Beas-2B, a bronchial epithelial cell line with TDI-HSA conjugate and compared with those without conjugate. IL-8 in the supernatant was measured by ELISA. To evaluate the effect of proinflammatory cytokines, peripheral blood mononuclear cells (PBMC) were collected from TDI- and non-TDI asthma patients, and were added to the epithelial cell culture. Dexamethasone or antibodies to TNF-alpha and IL-1beta were pre-incubated with PBMC supernatant. There was a significant production of IL-8 from bronchial epithelial cells with addition of TDI-HSA conjugate in a dose-dependent manner, which was significantly augmented with addition of PBMC supernatant. Higher production of IL-8 was noted with addition of PBMC supernatant from TDI-asthma patients than in those from non-TDI asthma patients. IL-1beta and IL-1beta/TFNalpha antibodies were able to suppress the IL-8 productions. Pre-treatment of dexamethasone induced dose-dependent inhibition of the IL-8 production. These results suggest that the IL-8 production from bronchial epithelial cells contribute to neutrophil recruitment occurring in TDIinduced airway inflammation. IL-1beta released from PBMC of TDI-induced asthma patients may be one of the pro-inflammatory cytokines to enhance IL-8 production.

Keyword

Isocyanates; TDI-Asthma; Epithelial Cells; Interleukin-8; Interleukin-1; Dexamethasone

MeSH Terms

Asthma/immunology
Bronchi/*cytology/metabolism
Cell Line
Dexamethasone/pharmacology
Epithelial Cells/cytology/*drug effects/*metabolism
Glucocorticoids/pharmacology
Human
Interleukin-8/*metabolism
Leukocytes, Mononuclear/cytology/metabolism
Support, Non-U.S. Gov't
Toluene 2,4-Diisocyanate/*toxicity