J Asthma Allergy Clin Immunol.
2001 Feb;21(1):21-27.
The effect of hydrocortisone and interleukin 4 on allergen-specific IgE production by peripheral blood mononuclear cells from atopic patients
- Affiliations
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- 1Dept. of Internal Medicine, Ewha Womans University College of Medicine.
- 2Dept. of Pediatrics, University of Ulsan College of Medicine, Seoul, Korea.
- 3Dept. of Internal Medicine, University of Ulsan College of Medicine, Seoul, Korea. hbmoon@www.amc.seoul.kr
Abstract
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BACKGROUND: Although the basic cellular and molecular requirements for the induction and synthesis of whole human IgE antibody have extensively been investigated, the mechanisms involved in the regulation of allergen-specific IgE synthesis are not yet fully understood.
OBJECTIVES
The aims of this study were to elucidate the differences in the total and specific IgE regulation by hydrocortisone(HC) with interleukin 4 (IL-4) between atopics and non-atopics and to determine the relationship between in vitro IgE synthesis and serum IgE levels.
METHODS
Peripheral blood mononuclear cells(PBMCs) from sixteen atopic asthma patients sensitive to Dermatophagoides farinae(D.f) and seven non-atopics were cultured with IL-4 and/or HC. Total and D.f-specific IgE in culture supernatant were measured using ELISA and FAST methods respectively.
RESULTS
PBMCs from 8 of 16 atopics produced D.f-specific IgE in vitro either spontaneously or by IL-4 and/or HC. HC had more profound effects than IL-4 in these patients. They also showed higher total IgE synthesis by HC, and higher specific serum IgE levels than the others. IL-4 and/or HC did not induce any D.f-specific IgE synthesis by PBMCs from non-atopics.
CONCLUSION
These data suggest that atopic patients have allergen-specific B cells that have already been switched to IgE production, probably due to in vivo priming effect of IL-4.
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