J Asthma Allergy Clin Immunol.  2003 Jun;23(2):366-371.

Antagonism of sophoricoside from sophorica japonica on GM-CSF-induced cosinophil activation

• Yuan XZ
• Kim Y
• Jung SH
• Lee SH
• Ryu JC
• Kim MK

Abstract

BACKGROUND
allergic disease is an inflammatory disease, whose main inflammatory cells are eosinophils, mast cells, and T lymphocytes. From that point, new therapeutic targets on allergic inflammation focusing on them are under investigation. We extracted four isoflavonoids from sophorica japonica such as sophi, orobol, genistin and genistein which are known PTK antagonists. We documented that these iso-flavonoids except genistein had an antagonism on IL-5 and IL-3 in vitro eosinophil activation and also in allergic mouse model sensitized by OA(ovualbumin). Their common action is due to the common beta chains. GM-CSF also share common beta chains, through which it activates eosinophils.
OBJECTIVES
From the above results, We observed the antagonistic effects of these compounds on GM-CSF using eosinophil activation in vitro. METHODS: LTC4 which was detected by RIA and ECP by UniCAP were activation markers. RESULTS: Among those compounds, sophi was the most potent antagonist on GM-CSF induced LTC4 release and even on degranulation and orobol and genistin also had antagonism on them but genistein an antagonist of PTK did not show any antagonistic effects. CONCLUSION: From these results, We concluded these three iso-flavonoids were GM-CSF antagonists and the mechanism might not be through PTK signaling.

Keyword

Sophoricoside; GM-CSF; Eosinophil; Antagonist

MeSH Terms

Animals
Eosinophils
Genistein
Granulocyte-Macrophage Colony-Stimulating Factor
Inflammation
Interleukin-3
Interleukin-5
Leukotriene C4
Mast Cells
Mice
T-Lymphocytes
Genistein
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-3
Interleukin-5
Leukotriene C4