J Asthma Allergy Clin Immunol.  2003 Sep;23(3):459-466.

Antagonism of Sophoricoside from Sophorica japonica on IL-5

• Zhe YX
• Kim Y
• Jung SH
• Lee SH
• Ryu JC
• Kim MK

Abstract

BACKGROUND
Allergic disease is an inflammatory disease, whose main inflammatory cells are eosinophils, mast cells, and T lymphocytes. From that point, new therapeutic targets on allergic inflammation focusing on them are under investigation. We extracted four isoflavonoids from Sophorica japonica such as sophi, orobol, genistin, and genistein which has been known as PTK antagonist. We documented those three iso-flavonoids except genistein had an anti-inflammatory effect as potent as dexamethasone on carageen-induced ear model. Also they had antagonism on the Y-16 cell line, whose growth is dependent on IL-5.
OBJECTIVES
From above results, in this experiment, we tried to find antagonistic effects of those compounds on IL-5 using the inhibition of eosinophil activation and survival in vitro and possibility of anti-allergic medicine. METHODS: LTC4 by RIA and ECP for degranulation by UniCAP(TM), which had been used previously were activation markers. RESULTS: Among those compounds, sophi was the most potent antagonist on IL-5 induced LTC4 release, degranulation, and survival. Orobol and genistin also had antagonism on them, but genistein, an antagonist of PTK didn't show any antagonistic effects. CONCLUSION: From these results, we concluded those three iso-flavonoids were IL-5 antagonist, and the mechanism of it might not be through PTK signaling.

Keyword

Sophoricoside; IL-5; eosinophil; antagonist

MeSH Terms

Cell Line
Dexamethasone
Ear
Eosinophils
Genistein
Inflammation
Interleukin-5*
Leukotriene C4
Mast Cells
T-Lymphocytes
Dexamethasone
Genistein
Interleukin-5
Leukotriene C4