Korean J Physiol Pharmacol.  2015 Sep;19(5):427-434. 10.4196/kjpp.2015.19.5.427.

Functional Connections of the Vestibulo-spino-adrenal Axis in the Control of Blood Pressure Via the Vestibulosympathetic Reflex in Conscious Rats

Affiliations
  • 1Department of Physiology and Pathophysiology, Yanbian University College of Medicine and Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules at Yanbian University, Yanji 133002, China. y-z-jin@ybu.edu.cn
  • 2Department of Physiology, Wonkwang University School of Medicine and Brain Science Institute at Wonkwang University, Iksan 570-749, Korea. byungp@wku.ac.kr

Abstract

Significant evidence supports the role of the vestibular system in the regulation of blood pressure during postural movements. In the present study, the role of the vestibulo-spino-adrenal (VSA) axis in the modulation of blood pressure via the vestibulosympathetic reflex was clarified by immunohistochemical and enzyme immunoassay methods in conscious rats with sinoaortic denervation. Expression of c-Fos protein in the intermediolateral cell column of the middle thoracic spinal regions and blood epinephrine levels were investigated, following microinjection of glutamate receptor agonists or antagonists into the medial vestibular nucleus (MVN) and/or sodium nitroprusside (SNP)-induced hypotension. Both microinjection of glutamate receptor agonists (NMDA and AMPA) into the MVN or rostral ventrolateral medullary nucleus (RVLM) and SNP-induced hypotension led to increased number of c-Fos positive neurons in the intermediolateral cell column of the middle thoracic spinal regions and increased blood epinephrine levels. Pretreatment with microinjection of glutamate receptor antagonists (MK-801 and CNQX) into the MVN or RVLM prevented the increased number of c-Fos positive neurons resulting from SNP-induced hypotension, and reversed the increased blood epinephrine levels. These results indicate that the VSA axis may be a key component of the pathway used by the vestibulosympathetic reflex to maintain blood pressure during postural movements.

Keyword

c-Fos protein; Epinephrine; Glutamate; Intermediolateral cell column; Vestibulo-spino-adrenal axis; Vestibulosympathetic reflex

MeSH Terms

Animals
Axis, Cervical Vertebra*
Blood Pressure*
Denervation
Epinephrine
Excitatory Amino Acid Antagonists
Glutamic Acid
Hypotension
Immunoenzyme Techniques
Microinjections
Neurons
Nitroprusside
Rats*
Receptors, Glutamate
Reflex*
Vestibular Nuclei
Natural Resources
Epinephrine
Excitatory Amino Acid Antagonists
Glutamic Acid
Nitroprusside
Receptors, Glutamate

Figure

  • Fig. 1 Photomicrographs showing the effect of medial vestibular nucleus (MVN) glutamate on c-Fos protein expression in the spinal cord. (A) Effect of microinjection of glutamate receptor agonists into the left MVN on c-Fos protein expression in the intermediolateral cell column (IMC) of the T7 spinal cord. (B) Effect of pretreatment with microinjection of glutamate receptor antagonists into the left MVN on c-Fos protein expression in the IMC of the T7 spinal cord. Right lower quadrant in A and B represents higher magnification. Rectangles in right lower corner of each diagram represent higher magnification. ACSF, microinjection of artificial cerebrospinal fluid into the MVN; NMDA, microinjection of NMDA into the MVN; AMPA, microinjection of AMPA into the MVN; ACSF+SNP, SNP infusion after pretreatment with ACSF in the MVN; MK801+SNP, SNP infusion after pretreatment with MK801 in the MVN; CNQX+SNP, SNP infusion after pretreatment with CNQX in the MVN. ACSF, artificial cerebrospinal fluid; AMPA, 2-amino-3-(5-methyl-3-oxo-1,2-oxazol-4-yl) propanoic acid; CNQX, 6-cyano-7-nitroquinoxaline-2,3-dione; IMC, intermediolateral cell column; MK801, (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d] cyclohepten-5,10-imine maleate; NMDA, N-methyl-D-aspartic acid; SNP, sodium nitroprusside.

  • Fig. 2 Bar histogram showing the effect of medial vestibular nucleus (MVN) glutamate on c-Fos protein expression in the spinal cord. ACSF, microinjection of artificial cerebrospinal fluid into the MVN; NMDA, microinjection of NMDA into the MVN; AMPA, microinjection of AMPA into the MVN; ACSF+SNP, SNP infusion after pretreatment with ACSF in the MVN; MK801+SNP, SNP infusion after pretreatment with MK801 in the MVN; CNQX+SNP, SNP infusion after pretreatment with CNQX in the MVN. The number of animals in each group was 7. Values are mean±SE. *Significant difference from ACSF group (**p<0.01). †Significant difference from ACSF + SNP group (‡p<0.01). Other notations are as in Fig. 1.

  • Fig. 3 Photomicrographs showing the effect of rostral ventrolateral medullary nucleus (RVLM) glutamate on c-Fos protein expression in the spinal cord. (A) Effect of microinjection of glutamate receptor agonists into the right RVLM on c-Fos protein expression in the IMC of the T7 spinal cord. (B) Effect of pretreatment with microinjection of glutamate receptor antagonists into the right RVLM on c-Fos protein expression in the IMC of the T7 spinal cord. Right lower quadrant in A and B represents higher magnification. Rectangles in right lower corner of each diagram represent higher magnification. ACSF, microinjection of artificial cerebrospinal fluid into the RVLM; NMDA, microinjection of NMDA into the RVLM; AMPA, microinjection of AMPA into the RVLM; ACSF+SNP, SNP infusion after pretreatment with ACSF in the RVLM; MK801+SNP, SNP infusion after pretreatment with MK801 in the RVLM; CNQX+SNP, SNP infusion after pretreatment with CNQX in the RVLM. Other notations are as in Fig. 1.

  • Fig. 4 Bar histogram showing the effect of rostral ventrolateral medullary nucleus (RVLM) glutamate on c-Fos protein expression in the spinal cord. ACSF, microinjection of artificial cerebrospinal fluid into the RVLM; NMDA, microinjection of NMDA into the RVLM; AMPA, microinjection of AMPA into the RVLM; ACSF+SNP, SNP infusion after pretreatment with ACSF in the RVLM; MK801+SNP, SNP infusion after pretreatment with MK801 in the RVLM; CNQX+SNP, SNP infusion after pretreatment with CNQX in the RVLM. The number of animals in each group was 7. Values are mean±SE. *Significant difference from ACSF group (**p<0.01). †Significant difference from ACSF+SNP group (‡p<0.01). Other notations are as in Fig. 1.

  • Fig. 5 The effect of glutamate on levels of blood epinephrine. (A) Effect of microinjection of glutamate receptor agonists or antagonists into the right medial vestibular nucleus (MVN) on levels of blood epinephrine. (B) Effect of microinjection of glutamate receptor agonists or antagonists into the right rostral ventrolateral medullary nucleus (RVLM) on levels of blood epinephrine. The number of animals in each group was 7. Values are mean±SE. *Significant difference from ACSF group (**p<0.01). †Significant difference from ACSF+SNP group (‡p<0.01). Other notations are as in previous figures.

  • Fig. 6 The effect of microinjection of glutamate receptor agonists into the MVN on blood pressure. Microinjection of AMPA or NMDA into the MVN increased blood pressure, but blood pressure was not changed following ACSF microinjection. AMPA or NMDA was injected into the right MVN at a concentration of 1.5 mmol/L and a volume of 10 µL. Arrows indicate microinjection of ACSF and glutamate agonists.

  • Fig. 7 Schematic diagram of the vestibulo-spino-adrenal (VSA) axis in the vestibulosympathetic reflex. Dotted lines represent pathway of the VSA axis. VNC, vestibular nuclear complex; NTS, nucleus tractus solitarius; CVLM, caudal ventrolateral medulla; RVLM, rostral ventrolateral medulla; IML, intermediolateral cell column of the spinal cord; AM, adrenal medulla.


Cited by  1 articles

Role of peripheral vestibular receptors in the control of blood pressure following hypotension
Guang-Shi Jin, Xiang-Lan Li, Yuan-Zhe Jin, Min Sun Kim, Byung Rim Park
Korean J Physiol Pharmacol. 2018;22(4):363-368.    doi: 10.4196/kjpp.2018.22.4.363.


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