Intest Res.  2013 Oct;11(4):261-267. 10.5217/ir.2013.11.4.261.

Epigallocatechin-3-gallate Inhibits the Expression of Adhesion Molecules by Blocking Nuclear Factor Kappa B Signaling in Intestinal Epithelial Cells

• Myung DS
• Park YL
• Joo SY
• Myung E
• Chung CY
• Park HC
• Kim JS
• Cho SB
• Lee WS
• Kim HS
• Joo YE

• KMID: 2284913
• DOI: http://doi.org/10.5217/ir.2013.11.4.261

Abstract

BACKGROUND/AIMS
Epigallocatechin-3-gallate (EGCG) is the main polyphenol in green tea and has anti-inflammatory and anti-oxidative effects. The aim of this study was to determine the impact of EGCG on the expression of adhesion molecules and lipopolysaccharide (LPS)-induced nuclear factor-kappa B (NF-kappaB) signaling in rat intestinal epithelial (RIE) cells.
METHODS
The effect of EGCG on LPS-induced NF-kappaB signaling and expression of intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 was examined by reverse transcription polymerase chain reaction, western blotting, immunofluorescence and electrophoretic mobility shift assay.
RESULTS
LPS-induced expression of ICAM-1 and VCAM-1 mRNA was inhibited by EGCG treatment in RIE cells. LPS-induced inhibitor of kappa B alpha degradation and NF-kappaB nuclear translocation were blocked by EGCG in RIE cells. EGCG blocked LPS-induced NF-kappaB DNA-binding activity in RIE cells. The pharmacological NF-kappaB inhibitor Bay11-7082 suppressed the LPS-induced expression of ICAM-1 and VCAM-1 mRNA in RIE cells.
CONCLUSIONS
These results indicate that EGCG inhibits LPS-induced ICAM-1 and VCAM-1 expression by blocking NF-kappaB signaling in intestinal epithelial cells.

Keyword

Epigallocatechin gallate; Nuclear factor kappa B; Adhesion molecule; Intestinal epithelial cell

MeSH Terms

Animals
Blotting, Western
Catechin
Epithelial Cells*
Fluorescent Antibody Technique
Intercellular Adhesion Molecule-1
NF-kappa B*
Nitriles
Polymerase Chain Reaction
Rats
Reverse Transcription
RNA, Messenger
Sulfones
Tea
Vascular Cell Adhesion Molecule-1
Catechin
Intercellular Adhesion Molecule-1
NF-kappa B
Nitriles
RNA, Messenger
Sulfones
Tea
Vascular Cell Adhesion Molecule-1