Korean J Pediatr.  2009 Jun;52(6):667-673. 10.3345/kjp.2009.52.6.667.

Toll-like receptor 2 expression on monocytes from patients with BCG vaccine-associated suppurative lymphadenitis

• Oh HJ
• Shin KS

• KMID: 2281071
• DOI: http://doi.org/10.3345/kjp.2009.52.6.667

Abstract

PURPOSE
Toll-like receptor 2 (TLR2) is critical in the immune response to mycobacterial infections. The purpose of this study was to analyze TLR2 surface expressions and TLR2-mediated tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) production in patients with BCG vaccine-associated suppurative lymphadenitis. METHODS: Peripheral monocytes were separated from 16 patients with BCG vaccine-associated suppurative lymphadenitis and 10 healthy controls using a magnet cell isolation kit. Monocytes (1x10(6) cells/well) were incubated with a constant amount of Pam(3)CSK(4) (100 microgram/mL) for 24 hours. TLR2 surface expression on monocytes was analyzed by FACS analysis and TLR-2 mRNA expression was determined by RT-PCR. TLR2-mediated TNF-alpha and IL-6 production were measured by ELISA. RESULTS: In patients with BCG vaccine-associated suppurative lymphadenitis, low TLR2 expression on monocytes (3.39+/-1.2% versus 4.64+/-2.6%) together with significantly lower TLR2 mRNA expression than in the healthy controls was seen after Pam(3)CSK(4) stimulation. TLR2-mediated TNF-alpha and IL-6 production in patients with BCG vaccine-associated suppurative lymphadenitis (TNF-alpha, 775.5+/-60.8 pg/mL; IL-6, 4,645.8+/-583.9 pg/mL) were also lesser than that in healthy controls (TNF-alpha, 1,098.5+/-94.3 pg/mL; IL-6, 6,696.3+/-544.3 pg/mL). CONCLUSION: These findings suggest that low TLR2 expression on monocytes might be associated with increased susceptibility to BCG vaccine-associated suppurative lymphadenitis.

Keyword

Mycobacterium bovis bacillus Calmette-Gurin; BCG vaccine-associated suppurative lymphadenitis; Toll-like receptor 2; Polymorphism

MeSH Terms

Cell Separation
Enzyme-Linked Immunosorbent Assay
Humans
Interleukin-6
Lymphadenitis
Magnets
Monocytes
Mycobacterium bovis
RNA, Messenger
Toll-Like Receptor 2
Toll-Like Receptors
Tumor Necrosis Factor-alpha
Interleukin-6
RNA, Messenger
Toll-Like Receptor 2
Toll-Like Receptors
Tumor Necrosis Factor-alpha