Korean J Otolaryngol-Head Neck Surg.  2004 Mar;47(3):232-237.

Induction of Mucosal Tolerance Using House Dust Mite-Cholera Toxin B Conjugate in Mouse Model of Allergic Rhinitis: Prophylactic and Long-Term Therapeutic Effects

• Lee CH
• Oh SJ
• Kang JG
• Roh JL
• Lee SS
• Quan SH
• Rhee CS
• Min YG
• Sung SY

Abstract

BACKGROUND AND OBJECTIVES
Cholera toxin B (CTB) is an effective immunomodulating agent. Mucosal tolerance is a well recognized method for inducing tolerance, but large amounts of antigen is needed. The dosage required can be dramatically reduced through coupling the antigen to the CTB. This study aimed to examine whether mucosal administration of house dust mite coupled to CTB (HDM-CTB conjugate) would modulate specific type 1 hypersensitivity in the murine model of allergic rhinitis and to evaluate the prophylactic and long-term therapeutic effects. MATERIALS AND METHOD: C57BL/6 mice were sensitized with Dermatophagoides farinae extract. After administration of HDM-CTB conjugate, several parameters of allergic response were evaluated. RESULTS: After mucosal adminstration of HDM-CTB conjugate, allergic symptoms, eosinophilic infiltration into nasal mucosa, specific IgE, and the splenic T cell proliferation after Dermatophagoides farinae allergen challenge were suppressed. CONCLUSION: These findings show that HDM-CTB conjugate has an anti-allergic effect in the murine model of allergic rhinitis and suggest that house dust mite allergy can be modulated with mucosal administration of allergen coupled to CTB.

Keyword

Dermatophagoides farinae; Allergy; Cholera toxin; Immunotherapy

MeSH Terms

Administration, Mucosal
Animals
Cell Proliferation
Cholera Toxin
Dermatophagoides farinae
Dust*
Eosinophils
Hypersensitivity
Immunoglobulin E
Immunotherapy
Mice*
Nasal Mucosa
Pyroglyphidae
Rhinitis*
Cholera Toxin
Dust
Immunoglobulin E