Korean J Pediatr Gastroenterol Nutr.  1999 Mar;2(1):59-64.

Serum Fat Soluble Vitamins in Bile Duct Ligated Rats

• Sim JG
• O MH
• Kim KH

Abstract

PURPOSE: The aims of this study are to measure the serum levels of fat soluble vitamins (vitamin A and D) from bile duct ligated rats, and to evaluate the effect of oral bile acids administration to facilitate absorption of fat soluble vitamins. METHOD: We measured serum ALT, total bilirubin, vitamin A, and vitamin D of Sprague-Dawley rats 1 week before and 4 weeks after experimental bile duct ligation. Rats were consisted with 3 groups. Group 2 had been find bile acids and group 3 ursodeoxycholic acid after operation for 4 weeks. Multi-vitamin was given to all groups.
RESULTS
1) Base line (mean value before duct ligation): ALT 74.2 IU, total bilirubin 0.26 mg/dL; vitamin D 13.01 ng/mL vitamin A 0.87 microgram/mL, total bile acids 25.16 micron mol/L. 2) Four weeks after ligation: ALT 100.7 IU, total bilirubin 2.58 mg/dL; vitamin D 7.89 ng/mL vitamin A 1.37 microgram/mL, total bile acids 278.22 micron mol/L. 3) 4 weeks after ligation, each group (group 1, group 2 and group 3) showed vitamin D (7.62, 8.10 and 7.99) ng/mL, vitamin A (1.68, 1.06 and 1.33) microgram/mL, total bile acids (233.17, 345.80 and 268.57) micron mol/L, which were statistically not significant.
CONCLUSION
Serum level of vitamin A is increased after bile duct ligation although vitamin D is decreased. Oral administration of bile acids does not affect the serum levels of vitamin A and D in bile duct ligated rats.

Keyword

Cholestasis; Bile acids; Rat; Vitamin D

MeSH Terms

Absorption
Administration, Oral
Animals
Bile Acids and Salts
Bile Ducts*
Bile*
Bilirubin
Cholestasis
Ligation
Rats*
Rats, Sprague-Dawley
Ursodeoxycholic Acid
Vitamin A
Vitamin D
Vitamins*
Bile Acids and Salts
Bilirubin
Ursodeoxycholic Acid
Vitamin A
Vitamin D
Vitamins