Interleukin-8 Expression on Cultural Normal Human Dermal Fibroblasts by Aging

Won, Young Ho; Kim, Dae Young; Lim, Mi Hae; Lee, Seung Chul; Chun, Inn Ki

Abstract

Aging skin result in a structural change like an epidermal and dermal atrophy, and a functional alteration on the secretory activities, inflammatory or immune mechanism. Dermal fibroblast is main cellular component in the dermis, and essential for structural integrity and physiological function of skin. Beside them, it also take a part on the inflammation and immune function as secreting actively various kind of cytokines. IL-8 is a potent chemotactic agent produced on the variety of cells such as monocytes, endothelial cells, epithelial cells, fibroblast. Until recent days, alteration of cytokine production with again was evaluated chiefly on the inflammatory cells, and remain controversial by reporters. The aim of this study is to see any alternation of IL-8 secretion from dermal fibroblast with aging. The dermal fibroblasts were cultured respectively in an elderly and young man, and a male infant. The IL-8 mRNA expressions assayed by RT-PCR were increased in 3 times by stimulations of TNF alpha, however there was not in any difference among fibroblast. The IL-8 secretions were assayed by ELISA on the supernatant of cultured dermal fibroblast, which in an unstimulated condition were not in any difference among them. But on the fibroblasts stimulated by TNF alpha(100, 500 U/ml), they were increased in all fibroblasts, and significantly higher at the elderly as 4.26+/-0.98 than the infant as 2.97+/-0.7(ng/ml)(p<0.05). The stimulation by PMA(phorbol 12-myristate 13-acetate) also revealed a similar result that a higher secreation in the elderly as 3.21+/-0.8 compared to the infant as 2.13+/-0.4(ng/ml). This study demonstrate that TNF alpha and PMA could include IL-8 secreation of fibroblasts without age difference, and cause an overproduction in elderly fibroblast compared to the infant.